胶原涂层对3D打印种植体表面生物相容性的影响  

Influence of collagen coating on the biocompatibility of three-dimensional printed implants

作　　者：李赛娜 康跻耀[2] 高建萍[2] 高毅[3] 罗元明[4] 张贵锋[2] 王明林[1] 

机构地区：[1]山东农业大学食品科学与工程学院,山东省泰安市271018 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京市100190 [3]南方医科大学珠江医院,广东省广州市510280 [4]中国科学院微生物研究所,北京市100190

出　　处：《中国组织工程研究》2017年第10期1558-1564,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然科学基金资助项目(2014A030312013);国家高技术研究发展计划(863)基金资助项目(2014AA022109)~~

摘　　要：背景:金属等惰性生物材料存在生物相容性差、组织结合力弱等问题,从而导致种植牙或骨植入物的使用效果较差。因此,如何增强惰性生物材料的生物相容性成为了近几年的研究热点。目的:对钛合金材料进行胶原覆层并评价其生物相容性。方法:以钛合金片(Ti6Al4V)为基质,Ⅰ型胶原和Ⅱ型胶原为覆层材料,3-氨基丙基三乙氧基硅烷(KH550)为偶联剂,对基于3D打印的钛合金片进行胶原覆层。将覆层材料用于小鼠前成骨细胞(MC-3T3-E1)培养,通过细胞培养效果评价其生物相容性,并通过细胞蛋白差异性识别比较不同类型胶原对细胞分化的影响。结果与结论:(1)当钛合金片(Φ10 cm)表面含氮量为8.41%时,Ⅰ型胶原覆层量为0.81 mg,Ⅱ型胶原覆层量为0.77 mg;(2)与空白钛合金片相比,经胶原覆层后的钛合金片细胞贴附状态更好,铺展面积更大,且细胞增殖速率更快,活性更强;(3)Ⅰ型胶原覆层材料培养的细胞多表达与基质合成有关的蛋白,而Ⅱ型胶原覆层材料培养的细胞多表达与矿化及钙盐沉积有关的蛋白;(4)结果表明,胶原覆层可提高钛合金材料的生物相容性,且不同胶原类型可影响细胞表达不同功能性蛋白。BACKGROUND： Inert biomaterials such as metal usually hold poor biocompatibility and weak bonding force, which is against the effect of dental or bone implants. Therefore, how to improve their biocompatibility has become the research hotspot.OBJECTIVE： To prepare collagen-coated titanium alloy （Ti6Al4V）, and to assess its biocompatibility.METHODS： Ti6Al4V served as the matrix, the 3-amino propyl triethoxy silane （KH550） as the crosslinking agent, and the three-dimensional printed titanium alloy coated by collagen type Ⅰ and Ⅱ was prepared,respectively. The coated materials were co-cultured with mouse preosteoblasts MC-3T3-E1 to evaluate its biocompatibility. The effect of different kinds of collagen on the cell differentiation was compared by differential recognition of surface proteins.RESULTS AND CONCLUSION： When the N content on the titanium alloy surface （Φ10 cm） was 8.41%, the cladding quantity of collagen type Ⅰ and Ⅱ was 0.81 and 0.77 mg, respectively. Compared with the bare titanium alloy, the cell adhered well and distributed extensively on the coated titanium alloy,which showed strong viability and fast proliferation.The cells cultured on collagen type Ⅰ coated materials expressed the proteins associated with matrix synthesis, and those on collagen type Ⅱ coated materials expressed the proteins associated with mineralization. These results clarify that the collagen coating can improve the biocompatibility of titanium alloy, and different types of collagens act on different functional proteins.

关 键 词：生物材料 材料相容性 钛合金 胶原 种植体 生物相容性 成骨细胞 细胞蛋白 液质联用 广东省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]