姜黄素对H22肝癌、S180肉瘤小鼠的抑瘤作用及机制研究  

Anti-tumor effect and mechanism of curcumin on H22 hepatocellular carcinoma and S180 sarcoma mice

作　　者：刘碧霞[1] 章红燕[2] 

机构地区：[1]浙江省肿瘤医院腹部肿瘤内科,浙江杭州310022 [2]浙江省肿瘤医院药剂科,浙江杭州310022

出　　处：《中国现代医生》2017年第9期48-52,共5页China Modern Doctor

基　　金：浙江省中医药科技计划项目(2015ZQ008)

摘　　要：目的探讨姜黄素对H22肝癌、S180肉瘤小鼠的抑瘤作用及机制,为H22肝癌临床治疗提供实验依据。方法采用ICR小鼠接种H22瘤株、S180肉瘤株分别建立肿瘤模型,采用姜黄素进行干预,明确其抑瘤作用,分阳性对照组(CTX化疗组)、阴性对照组(生理盐水组)、姜黄素低剂量组、姜黄素中剂量组、姜黄素高剂量组,每组12只。对比分析治疗组各组用药前后体重、抑瘤率的差异;动物处死后,取肿瘤组织,采用荧光定量RT-PCR测定肿瘤组织中TGF-β1RⅡ、NF-κB、CyclinD1、VEGF表达。结果姜黄素对肝癌和肉瘤小鼠肿瘤生长抑制率为36.04%和41.27%,有明显的抑瘤作用。与模型组比较,给药组肿瘤组织NF-κB、CyclinD1、VEGF的表达水平下调,TGF-β1RⅡ的表达水平显著升高。结论姜黄素通过上调肿瘤组织TGF-β1RⅡ,下调NF-κB、CyclinD1、VEGF表达水平抑制肿瘤细胞增殖,抗血管新生,实现抑制肿瘤作用。Objective To investigate the anti-tumor effect and mechanism of curcumin on H22 hepatocellular carcinoma and S180 sarcoma mice, and to provide experimental evidence for the clinical treatment of H22 hepatocellular earcinoma. Methods Tumor models were established respectively by inoculating ICR mice with H22 tumor strain and S180 sarcoma strain.Curcumin was used to intervene, and its anti-tumor effect was identified.The mice were divided into the positive control group（CTX chemotherapy group）, negative control group （saline group）, low-dose curcumin group, middle-dose cureumin group and high-dose eurcumin group, with 12 mice in each group. The difference of body weight before and after treatment and tumor inhibition rate among groups in the treatment group was comparatively analyzed. The tumor tissue was taken after the animals were sacrificed. The expression of TGF-β1RⅡ, NF-κB, CyclinD1 and VEGF in tumor tissue were measured by fluorescence quantitative RT-PCR. Results The inhibitory rate of curcumin on tumor growth was 36.04% and 41.27% in hepatoeellular carcinoma and sarcoma mice, with obvious anti-tumor effect. Compared with that of the model group, the expression of NF-κB, CyclinD1 and VEGF in the tumor tissue was down-regulated and the expression of TGF-β1R Ⅱ was significantly increased. Conclusion Curcumin can inhibit the proliferation of tumor cells and angiogenesis to achieve the effect of tumor inhibition by up-regulating TGF-β1R Ⅱ and down-regulating the expression of NF-κB, CyclinD1 and VEGF in tumor tissue.

关 键 词：姜黄素 H22肝癌 S180肉瘤 抑瘤作用 机制研究 

分 类 号：R285.5[医药卫生—中药学]