佐剂性关节炎模型兔成纤维样滑膜细胞的培养  

Culture of fibroblast-like synoviocytes from rabbits with adjuvant arthritis

作　　者：王丽平[1] 刘琴[1] 陈芳[1] 陈利锋[2] 张宜[1] 

机构地区：[1]中国人民解放军武汉总医院医学实验科,湖北省武汉市430070 [2]中国人民解放军武汉总医院中西结合科,湖北省武汉市430070

出　　处：《中国组织工程研究》2017年第12期1872-1876,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:目前研究者多采用酶消化法从类风湿性患者和佐剂性关节炎大鼠的滑膜组织中分离培养成纤维样滑膜细胞,采用组织块贴壁法体外培养佐剂性关节炎兔成纤维样滑膜细胞较少见。目的:建立佐剂性关节炎兔成纤维样滑膜细胞的培养方法并观察细胞的生物学特性。方法:采用多点多次注射佐剂的方法制备佐剂性关节炎兔动物模型,取膝关节滑膜组织,用组织块贴壁法分离培养成纤维样滑膜细胞,观察细胞形态特征。采用CCK-8法检测细胞增殖情况,并用免疫荧光法检测波形蛋白的表达。结果与结论:倒置显微镜观察原代培养的关节炎兔滑膜细胞形态均为长梭状纤维样,符合成纤维样滑膜细胞的生长特征,且表达波形蛋白的细胞达98%。说明采用组织块贴壁法成功分离培养了佐剂性关节炎兔成纤维样滑膜细胞。BACKGROUND：Currently, fibroblast-like synoviocytes from the synovial tissue of patients with rheumatoid arthritis or adjuvant arthritis rats are isolated and cultured mainly using enzyme digestion method, but tissue explant adherent method is rarely reported in primary culture. OBJECTIVE：To establish a culture method of fibroblast-like synoviocytes from adjuvant arthritis rabbits and to investigate cell biological characteristics. METHODS：Rabbit models of adjuvant arthritis were prepared by injected with adjuvant at multiple sites and times, the knee synovial tissues were obtained, and fibroblast-like synoviocytes were separated and cultured by the tissue explant adherent method. Furthermore, the cell morphology was observed. The cell proliferation was measured using cell counting kit-8 assay, and the expression of Vimentin was detected by immunofluorescence.RESULTS AND CONCLUSION：The primary cultured rabbit synoviocytes were long spindle fiber-like and coincided with the fibroblast-like synoviocytes. Moreover, cells were positive for Vimentin. In conclusion, fibroblast-like synoviocytes from rabbits with adjuvant arthritis are successfully isolated and cultured by the tissue explant method.

关 键 词：组织构建 组织工程 佐剂性关节炎 兔 成纤维样滑膜细胞 组织块贴壁法 

分 类 号：R593.22[医药卫生—内科学]