新疆红花对皮肤细胞紫外线辐射损伤的保护与修复功能研究  

Protective and Repairing Effects of Xinjiang Safflower on Ultraviolet Radiation Damage of Skin Cells

作　　者：陈鋆[1] 余灵凤 蔡淑萍[1] 

机构地区：[1]金华职业技术学院,浙江金华321007

出　　处：《金华职业技术学院学报》2017年第3期63-68,共6页Journal of Jinhua Polytechnic

基　　金：浙江省2016年新苗人才计划立项"艾叶-红花皮肤修复功能研究及抗皲裂膏的开发"(2016R468007)

摘　　要：研究新疆红花总黄酮(红花总黄酮)的提取工艺及其对CCD966SK细胞受紫外线损伤的保护和修复功能。采用单因素实验对红花总黄酮的提取工艺进行探索,并以紫外线照射伤害方式考察红花总黄酮对培养的CCD966SK细胞的修复和保护能力。结果显示:新疆红花与60%乙醇溶液料液比为1:12,回流提取1.5 h,重复提取3次,红花总黄酮提取率可达12.83 mg/g;当红花总黄酮浓度为500μg/m L时,红花总黄酮对CCD966SK细胞有良好的紫外线损伤的保护和修复功能。The extraction technology of total flavonoids from safflower （Safflower total flavonoids） and the protective and repairing effects on CCD966SK cells by UV-ray are studied. The extraction of Safflower total flavonoids is studied by single factor experiment, and the repairing and protective effects of Safflower total flavonoids on CCD966SK cells are investigated by UV-irradiation. The results show that the extraction condition is： ratio of Xinjiang safflower to 60% ethanol is 1 ： 12, 1.5 hour of reflux, and 3 times of extraction respectively; when the concentration of safflower total flavonoids is 500 μg/mL, it has a good orotective and reoairing effects on UV damage of the CCD966SK cells.

关 键 词：红花 总黄酮 羟基红花黄色素A 人类皮肤纤维母细胞 紫外线 辐射防护 

分 类 号：TQ460.7[化学工程—制药化工]