姜黄素对人肾癌细胞786-0体外增殖的抑制作用  

Inhibitory Effect of Curcumin on Proliferation of Human Renal Carcinoma Cell Line 786-0 in Vitro

作　　者：赵建通[1] 张磊[1] 刘文瞻[1] 徐飞[1] 郭明涛[1] 路志民[1] 肖波[1] Zhao Jiantong Zhang Lei Liu Wenzhan et al(The First Hospital of Handan , Handan , Hebei 056000, Chin)

机构地区：[1]邯郸市第一医院泌尿一科,河北邯郸056000

出　　处：《四川医学》2017年第4期389-391,共3页Sichuan Medical Journal

摘　　要：目的观察姜黄素对人肾癌细胞786-0体外增殖的抑制作用。方法采用MTT法测定细胞增殖。结果由6.25μmol/L、12.5μmol/L、25μmol/L、50μmol/L的姜黄素处理24h、48h、72h后,786-0细胞生长均受到不同程度地抑制,与对照组相比各处理组OD值均有不同程度下降,差异有统计学意义(P<0.05),各处理组间比较差异亦有统计学意义(P<0.05)。不同浓度姜黄素(6.25μmol/L、12.5μmol/L、25μmol/L、50μmol/L)作用786-0细胞24h、48h、72h后抑制率分别变化为8.1%~58.0%、9.4%~67.7%、13.1%~75.3%,与对照组相比差异有统计学意义(P<0.05)。各处理组PI值均小于对照组,且随姜黄素素浓度增大而逐渐减小,与对照组比较,差异有统计学意义(P<0.05)。结论姜黄素能有效地抑制激素人肾癌细胞786-0增殖,姜黄素有可能成为一种治疗肾细胞癌的靶向新药。Objective To observe the inhibitory effect of curcumin on the proliferation of human renal carcinoma cell line786-0 in vitro. Methods Cell proliferation was measured by MTT assay. Results After24 h,48h,72 h treated by curcumin of 6. 25mol/L,12. 5 mol/L,25 mol/L,50 mol/L,growth of 786-0 cell was inhibited,compared with the control group,the OD values of all treatment groups were decreased,the difference was statistically significant（ P〈0. 05）. And there was also significant difference between the treatment groups（ P〈0. 05）. After 24 h,48h,72 h different concentrations of curcumin（ 6. 25 mol/L,12. 5 mol/L,25mol/L,50 mol/L） on 786-0 cells,changesof inhibition rate were respectively8. 1% ～ 58%,9. 4% ～ 67. 7%,13. 1% ～ 75. 3%,compared with the control group the difference was statistically significant（ P〈0. 05）. The PI values of each treatment group were lower than those of the control group,and decreased with the increase of curcumin concentration. Compared with the control group,the difference was statistically significant（ P〈0. 05）. Conclusion Curcumin can effectivelyinhibit the proliferation of human renal carcinoma cell line 786-0,which may be a new target for the treatment of renal cell carcinoma.

关 键 词：姜黄素 肾癌 786-0 细胞增殖 

分 类 号：R285[医药卫生—中药学]