氯虫苯甲酰胺亚致死剂量对甜菜夜蛾主要解毒酶活性与生长繁殖的影响  

Effects of Sublethal Doses of Chlorantraniliprole on the Detoxification Enzymes Activities and the Growth and Reproduction of Spodoptera exigua

作　　者：陈羿渠 向兴[1] 贡常委 王学贵[1] 

机构地区：[1]四川农业大学农学院无公害农药研究实验室,成都611130

出　　处：《中国农业科学》2017年第8期1440-1451,共12页Scientia Agricultura Sinica

基　　金：国家公益性行业(农业)科研专项(201203038)

摘　　要：【目的】甜菜夜蛾(Spodoptera exigua)是一种杂食性害虫,在不同环境、药物等选择压力下表现出不同的生长发育特点。氯虫苯甲酰胺是一种新型广谱的鱼尼丁受体杀虫剂,对鳞翅目害虫杀虫活性强。本研究旨在探究氯虫苯甲酰胺亚致死剂量对甜菜夜蛾幼虫3种主要解毒酶——羧酸酯酶(Car E)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFOs)活性以及对种群繁殖的影响。【方法】采用饲料混毒法测定氯虫苯甲酰胺对SE-Lab品系、SE-Sel品系的毒力,SE-Sel品系由SE-Lab品系经亚致死剂量LC25连续汰选6代得到;通过浸叶法测定磷酸三苯酯(TPP)、顺丁烯二酸二乙酯(DEM)、胡椒基丁醚(PBO)3种酶抑制剂与氯虫苯甲酰胺协同对SE-Lab和SE-Sel品系的毒力增效作用,提前12 h让试虫取食浸渍过酶抑制剂的叶片,对照组取食用0.1%Triton X-100浸渍后的叶片,再分别测定氯虫苯甲酰胺对使用酶抑制剂与未使用酶抑制剂试虫的毒力;在冰上解剖试虫的中肠和脂肪体,并通过离体酶活性测定,分析氯虫苯甲酰胺亚致死剂量与酶抑制剂对甜菜夜蛾体内的3种代谢解毒酶活力的影响;通过记录试虫各个年龄阶段的生长、死亡、产卵量等数据,参照两性生命表理论分析SE-Lab和SE-Sel品系的两性生命表参数差异。【结果】在3种酶抑制剂中PBO增效作用最强,其对甜菜夜蛾SE-Sel品系和SE-Lab品系对氯虫苯甲酰胺的毒力增效比分别为1.58和1.69。在氯虫苯甲酰胺亚致死剂量连续汰选下,甜菜夜蛾体内3种解毒酶活性均被诱导上升,其中MFOs酶活力上升最显著,SE-Sel品系中肠和脂肪体的MFOs活性相对于SE-Lab品系分别提高了2.07和2.10倍,而经氯虫苯甲酰胺亚致死剂量再次诱导的SE-Sel试虫的MFOs活性亦较SE-Lab品系上升4.02和3.44倍;在使用了酶抑制剂后3种解毒酶酶活力均有下降,其中MFOs活性下降最多,其酶比活力仅为未使用酶抑制剂处理的42.3%—44.8【Objective】 Spodoptera exigua is a polyphagous pest and shows different characteristics of growth and development under the selective pressure of different environments,drugs and so on.Chlorantraniliprole,a novel insecticide that acts on ryanodine receptors,has highly activity to those insects in Lepidopteran.The objective of this study is to explore the effects of S.exigua larvae treated by the sublethal doses of chlorantraniliprole on the toxicities,the activities of three main detoxifying enzymes,including carboxylesterase（Car E）,glutathione S-transferase（GSTs）,and mixed-function oxidase（MFOs）,and population breeding.【Method】 The toxicities of chlorantraniliprole on SE-Lab and SE-Sel strains were detected by incorporation bioassay and the SE-Sel strain was achieved with continuous selecting with LC25 sublethal doses for six generations from SE-Lab strain,then the synergistic effects of enzyme inhibitors（TPP,DEM,PBO） with chlorantraniliprole on the SE-Lab and SE-Sel strains were assayed using the dip-leaf method.The toxicities of chlorantraniliprole on the insects which were fed the leaves soaked with the solution of enzyme inhibitor or 0.1% Triton X-100 as blank control before 12 h were assayed by the dip-leaf method.The midgut and fatbody of tested insects were dissected on the ice and the effects of the sublethal doses of chlorantraniliprole and enzyme inhibitors on three metabolic detoxification enzyme activities were analyzed by the determination of detoxification enzyme activities.According to the age-stage two-sex life tables theory,the growth,mortality,fecundity data of the tested insects were also recorded to analyze the differences of the age-stage two-sex life table parameters between the strains of SE-Lab and SE-Sel.【Result】 The synergistic effect of the PBO was the strongest among the three enzyme inhibitors and the synergic ratios on the strains of SE-Sel and SE-Lab reached 1.58-and 1.69-fold,respectively.The activities of the three detoxification enzymes,which were ind

关 键 词：甜菜夜蛾 氯虫苯甲酰胺 亚致死剂量 增效剂 多功能氧化酶 两性生命表 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]