低温胁迫对黄姑鱼(Nibea albiflora)抗氧化酶、Na^＋/-K^＋-ATP酶及Hsp70 蛋白含量的影响  

Effects of low temperature stress on activities of antioxidant enzymes,Na^+/-K^+-ATP enzyme and Hsp70 content of Nibea albiflora

作　　者：罗胜玉 徐冬冬[2] 楼宝[2] 陈睿毅 詹炜[2] 毛国民[2] 

机构地区：[1]浙江海洋大学,浙江舟山316004 [2]浙江省海洋水产研究所,浙江省海水增养殖重点实验室,浙江舟山316100

出　　处：《海洋通报》2017年第2期189-194,共6页Marine Science Bulletin

基　　金：国家自然科学基金(41476127);浙江省科技计划项目(2012C12907-8;2015F10001)

摘　　要：为探讨低温处理对黄姑鱼幼鱼生理机能的影响,以18℃为对照组,设置8℃、10℃和14℃3个低温胁迫组,测定不同温度急性胁迫对黄姑鱼幼鱼(Nibea albiflora)超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、Na^+/-K^+-ATP酶活力以及热休克蛋白70(Hsp70)蛋白含量的影响。研究结果表明,低温胁迫组(8℃、10℃和14℃)的SOD和CAT酶活力随着胁迫温度的降低及其胁迫时间的延长呈先升高后降低的趋势,在72 h时恢复到对照组水平。肌肉中的Na^+/-K^+-ATP酶活力则随处理时间的延长呈现先降低后升高的趋势,10℃和14℃组能够在72 h时恢复到对照组水平,而8℃组则在72 h仍显著低于对照组。低温胁迫下黄姑鱼肌肉的Hsp70含量随着处理时间的延长呈先升高后降低趋势,10℃和14℃组能够在72 h时恢复到对照组水平,而8℃组则在72 h仍显著高于对照组。由此说明,SOD、CAT、Na^+/-K^+-ATP以及Hsp70蛋白参与了黄姑鱼低温胁迫应答过程,可以作为其低温胁迫应答的标志物。In order to investigate the effects of low temperature treatment on the physiological function of juvenile Nibea albiflora, we investigated the effect of cold stress on activities of antioxidant enzymes, Na^＋/-K^＋-ATP enzyme and HspT0 content in juvenile Nibea albiflora. Four treatments were set up, and 18 ℃ was as the control and 8 ℃, 10 ℃ and 14 ℃ groups were as experimental groups. We measured the activities of antioxidant enzymes, Na^＋ K^＋-ATP enzymes and HspT0 content of N. albiflora at different temperatures. The results showed that the temperature significantly influenced the SOD and CAT enzyme activities, and Na^＋/-K^＋-ATP enzyme activities and Hsp70 content of N. albiflora. The results showed that antioxidant enzyme activities increased in the beginning and then decreased under the low temperature stress, and at 72 h post-stress, antioxidant enzyme activities in low temperature treatments were at the same level as the control （P 〉 0.05 ） . Na^＋/-K^＋-ATP enzyme activities in the muscles of low temperature treatments showed the trend of decreasing firstly and then rising during the process of cold temperature stress. The Na^＋/-K^＋--ATP enzyme activities of 10 ℃ and 14 ℃ treatments could recover and have no significant differences at 72 h post-stress compared with the 18 ℃ treatment, but the activities of Na^＋/-K^＋-ATP enzyme in 8 ℃ treatment remained lower at 72 h post-stress. The HspT0 contents in different treatments were also impacted by the low temperature, and showed the trend of increasing firstly and then decreasing during the process of cold temperaturestress. Compared with the control, the Hsp70 contents of 10 ℃ and 14 ℃ treatments recovered and showed insignificant differences at 72 h post-stress. However, the HspT0 contents remained higher than that of control at 72 h post-stress. Therefore, SOD, CAT, Na^＋/-K＋-ATPase and Hsp70 were involved in the process when N. albiflora was responding to low temperature stress, and they could be the marker

关 键 词：微低温胁迫 黄姑鱼 SOD酶 CAT酶 Na^+/-K^+-ATP酶 HSP70 

分 类 号：P735[天文地球—海洋生物学]