不同温度对不同基因型棉花叶片叶绿素荧光的影响  

Effects of Different Temperatures on Chlorophyll Fluorescence of Different Genotypes of Cotton Leaves

作　　者：邢双涛[1] 李志博[1] 汤丽魁[1] 赵瑞海[1] 魏亦农[1] 

机构地区：[1]石河子大学农学院/新疆生产建设兵团绿洲生态农业重点实验室,新疆石河子832000

出　　处：《新疆农业科学》2017年第3期403-408,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科技支撑项目"高温对棉花光合特性;叶绿素荧光及抗氧化体系的影响"(31560406)~~

摘　　要：【目的】在不同温度条件下,研究不同基因型棉花叶片叶绿素荧光特性指标的变化,为耐高温棉花品种的筛选提供理论依据。【方法】分别在25、30、35、40、45和48℃的恒温培养箱处理不同基因型棉花13-1品系、新海24号、13-1×新海24号(杂交种)、13-1×新海14号(杂交种),利用快速荧光测定仪(Handy PEA-100)测定不同基因型棉花叶片荧光特性主要参数。【结果】随着温度不断升高,性能指数PIABS、最大光化学Fv/Fm、电子传递产额φEo表现为先升高后降低,而热耗散DIO/CSO、初始荧光FO则先降低后升高;性能指数PIABS在40℃时4个不同棉花基因型棉花品系呈极显著,比Fv/Fm更准确地反映光合状况。【结论】利用隶属函数法分析4种不同基因型棉花品种(系),其中13-1×新海24号隶属函数值最高,属于耐热品系。[ Objective ] This study aims to investigate the differences of leaf chlorophyll fluorescence characteristic parameters between different genotypes of cotton under different temperatures and to provide a theoretical basis to screen suitable indexes for evaluating high temperature - tolerance of cotton varieties. [ Method ] Chlorophyll fluorescence characteristic parameters in leaves of cotton seedlings of four different genotypes （ 13 - 1 strain, Xinhai 24, 13 - 1 × Xinhai 24 hybrid, 13 - 1 ×Xinhai 14 hybrid） were determined by rapid fluorescence detector （Handy PEA -100） under 25℃, 30℃, 35℃, 40℃, 45℃ and 48℃ respectively. [ Result] The performance index of PIABS, maximum photochemical Fv/Fm and electron transfer yield φEo increased at first and decreased subsequently as the temperature increased, the heat dissipation DIo/CSo , ini- tial fluorescence Fo was on the contrary. PIABs performance index between the four different genotypes of strains was extremely significant at 405℃ , and it could more accurately reflect photosynthetic status than Fv/ Fm. [ Conclusion] Using the method of subordinate function to analyze the four different genotypes, it is found that 13 -1 × Xinhai 24（hybrid） has the highest subordinate function value, and we believe that it belongs to heat resistant strains.

关 键 词：棉花 温度 最大光化学效率 性能指数 叶绿素荧光 

分 类 号：S562[农业科学—作物学]