卵巢去势对兔股骨皮质骨形态及生物力学影响  

Influences on morphology and biomechanics in rabbit＇ s femur cortex of ovariectomy

作　　者：黄海明[1] 李晓林[2] 涂淑强[1] 蒋梁华 陆长春[2] 陈晓峰[2] 

机构地区：[1]南昌市洪都中医院骨伤科,330006 [2]上海交通大学附属第六人民医院骨科,200233 [3]昆山市人民医院关节科,215300

出　　处：《中华骨科杂志》2017年第9期553-559,共7页Chinese Journal of Orthopaedics

基　　金：国家自然科学基金资助课题（81071501）;上海市科委医学引导项目（09411966500）

摘　　要：目的探讨卵巢去势对兔股骨皮质骨形态及生物力学的影响。方法新西兰系纯种大白兔80只，雌性，6月龄，体重（2．2±0．28）kg，分两组：去势组40只和假手术组40只。两组分别于术后4周和8周测试股骨皮质骨孔隙数量、孔隙体积、孔隙率、最大载荷，并行疲劳性骨微损伤实验，计算股骨皮质骨裂纹数量、长度及密度。结果Micro-CT检测发现，术后4周和8周去势组股骨皮质骨孔隙数量、孔隙体积、孔隙率均高于假手术组，且两组比较差异有统计学意义。生物力学检测显示，术后4周，假手术组和去势组股骨平均最大载荷分别为1949．25±53．12和1892．60±59．09，两组比较差异有统计学意义（P=0．003）；术后8周，两组平均载荷均下降，分别为1944．55±41．76和1692．40±85．08，两组比较差异有统计学意义（P=0．000）；去势组平均最大载荷下降较明显。骨微损伤实验显示，术后4周去势组股骨皮质骨裂纹的数量（3．40±1．67）、长度[（216．80±17．60）μm]及密度[（0．40±0．08）个／mm2]均明显高于假手术组数量（2．00±1．17）、长度[（160．45±16．89）μ]及密度[（0．29±0．13）个／mm。]；术后8周去势组股骨皮质骨裂纹的数量（5．15±1．18）、长度[（334．60±13．94）μm]及密度[（0．35±0．10）个／mm2]均明显高于假手术组数量（3．10±1.37）、长度[（182．10±9．80）μm]及密度[（0．24±0．09）个／mm2]，且同一时间点两组比较差异均有统计学意义。结论卵巢去势可增加兔皮质骨的孔隙率，降低其生物性能，加速疲劳性损伤，减缓骨修复；而皮质骨的变化可能成为引发骨折及骨折后延迟愈合极其重要的因素。Objective To investigate the influences on the femur cortex of the rabbit after ovariectomy and its mecha- nism. Methods Eighty 6 months-old female pure New Zealand rabbits were divided into two groups： 40 rabbits in ovariectomy group and 40 in sham-operation group. The weight averaged 2.2±0.28 kg. Four weeks and 8 weeks after operation, a series of tests were performed in both groups concerning the number, the volume, the rate and the maximal load of cortical bone porosity. The number, the length and the density of linear crack in rabbit femur cortex were documented after repetitive application of minor trauma. Results Micro-CT demonstrated that both on week 4 and 8 after operation, the number, the volume and the rate of corti- cal bone porosity were all significantly higher in ovariectomy group than that of the control group. Four weeks after operation, the biomechanical test showed the significantly lower average maximal load of rabbit femur in ovariectomy group （1 892.60±59.09） than that of in control group （1 949.25±53.12） （P=0.003）. Eight weeks after operation, the average load of both groups decreased to some extent, which was 1 944.55±41.76 in control group and 1 692.40±85.08 in ovariectomy group respectively （P=0.000）. Howev- er, the average maximal load of ovariectomy group decreased more significantly. Having application of repetitive minor trauma to the bone, the number, the length and the density of hnear crack of cortical bone were 3.40± 1.67, 216.80± 17.60 μm and 0.40±0.08/mm2 in ovariectomy group, and 2.00±1.17, 160.45±16.89 μm and 0.29±0.13/mm2 in control group 4 weeks later. And after 8 weeks, they were 5.15±1.18, 334.60±13.94 μm and 0.35±0.10/mm2 in ovariectomy group, and 3.10±1.37, 182.10±9.80 μm and 0.24± 0.09/mm2 in control group. The number, the length and the density of linear crack of cortical bone were all significantly higher in ovariectomy group than that of in control group both on week 4 and on week 8 after operation. Conclusion Ovariectomy incr

关 键 词：兔 卵巢切除术 股骨 生物力学 

分 类 号：R580[医药卫生—内分泌] R683[医药卫生—内科学]