定量蘸菌法鉴定向日葵品种黄萎病抗性  

Identification of sunflower verticillium wilt resistant with a quantitative method for dipping bacteria solution

作　　者：郭树春[1,2] 张艳芳[1] 孙瑞芬[1] 李素萍[1] 于海峰[1] 刘腊青 安玉麟[1] GUO Shuchun ZHANG Yanfang SUN Ruifen LI Suping YU Haifeng LIU Laqin AN Yulin(Inner Mongolia Academy of Agricultural ＆Animal Husbandry Sciences,Hohhot 010031,China Laboratory of Theoretical Biophysics, School of Physical Science and Technology, Inner Mongolia University, Hohhot 010021, China Helingeer Heilaoyao Township Agricultural Extension Service Station,Heilaoyao 011500, China)

机构地区：[1]内蒙古农牧业科学院,内蒙古呼和浩特010031 [2]内蒙古大学物理科学与技术学院,内蒙古呼和浩特010010 [3]和林格尔县黑老夭乡农技推广综合服务站,内蒙古黑老夭011500

出　　处：《北方农业学报》2017年第2期77-81,共5页Journal of Northern Agriculture

基　　金：国家向日葵产业技术体系项目(CARS-16);国家自然基金项目(31460234)

摘　　要：文章研究了一种向日葵黄萎病抗性室内鉴定法——定量蘸菌法,该方法与向日葵实际生长条件接近,可对向日葵黄萎病病程进行随时鉴定,通过该方法对抗黄萎病材料S18和易感黄萎病材料P77用高致病菌株侵染,致病性测定结果显示:抗黄萎病材料S18抗性明显高于易感黄萎病材料;生理生化指标测定结果显示:两个品种的可溶性蛋白含量和POD活性变化存在差异,抗病品种的可溶性蛋白含量总体高于感病品种,在黄萎病侵染下,抗病品种S18较感病品种P77 POD活性更强。a method about the identification of sunflower verticillium wilt resistance： a quantitative method for dipping bacteria solution was studied in this paper.This method is close to actual sunflower growth conditions, can be carried out at any time to identify the sunflower verticiflium wilt resistance through the method. Resistant （S18） and susceptible （P77） sunflower genotypes infected with V dahlia by this method. The results of pathogenic detection showed that resistance characteristics of resistance genotypes S l 8 was significantly higher than that of susceptible genotypes P77; physiological and biochemical index determination results showed that the change of soluble protein content and POD activity of two genotypes are different. Generally, the content of soluble protein was higher in resistance genotypes S18 than in susceptible genotypes P77. After infected with V dahlia, the POD had a stronger activity in resistance genotypes S 18 when compared with susceptible genotypes P77.

关 键 词：向日葵 向日葵黄萎病 抗性鉴定法 

分 类 号：S565.5[农业科学—作物学]