UPLC-PDA同时测定藏药翼首草环烯醚萜苷和酚酸类10个化学成分的含量  

Simultaneous determination of ten major compounds including iridoid glycosides and phenolic acids in Pterocephalus hookeri by UPLC-PDA

作　　者：唐策[1] 文检 王静[1] 赵可惠 范刚[1] 孟宪丽[1] 邹忠梅[2] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国中药杂志》2017年第7期1234-1237,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81274193);四川省教育厅属高校科研创新团队建设计划项目(11TD004)

摘　　要：建立超高效液相色谱法(UPLC-PDA)同时测定藏药翼首草环烯醚萜和酚酸类10个化学成分的含量。采用Acquity UPLC^R BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱,流动相0.2%磷酸水(A)-乙腈(B),梯度洗脱,检测波长237,325 nm,流速0.4 mL·min^(-1),柱温30℃,进样量1μL。研究结果表明UPLC-PDA法定量分析翼首草10个化学成分在考察的范围内线性关系良好(r≥0.999 7);加样回收率为96.30%~103.0%(n=9),RSD为0.72%~2.9%(n=9)。所建立UPLC方法简便、准确、重复性好,可同时测定藏药翼首草中10个化学成分的含量。This study is to develop an UPLC-PDA method for determination of 10 major components in Pterocephalus. The UPLC-PDA assay was performed on a Waters Acquity UPLCR BEH C18（2.1 mm×100 mm,1.7 μm）, and the column temperature was at 30℃. The mobile phase consists of water containing 0.2% phosphoric acid （A） and acetonitrile （B） in gradient elution at a flow rate of 0.4 mL·min^-1. The detection wave length was set at 237 and 325 nm, and the injection volume was 1 μL in the UPLC system. The linear range of 10 detected compounds were good （r≥0.999 7）, and the overall recoveries ranged from 96.30% to 103.0%, with the RSD ranging from 0.72% to 2.9%. The method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of ten major components in P. hookeri.

关 键 词：藏药 翼首草 UPLC-PDA 环烯醚萜 酚酸 含量测定 

分 类 号：R29[医药卫生—民族医学]