香水百合试管鳞茎形成与增殖条件优化研究  

Culture Condition Optimization of Test Tube-bulb Formation and Propagation of Lilium casablanca

作　　者：杨鹭生[1] 陈晓敏[1] 戚卉[1] 李娟[1] 李国平[1] YANG Lusheng CHEN Xiaoming QI Hui LI Juan LI Guoping(School of Ecology and Resource Engineering, Wuyi University, Wuyishan, Fujian 35430)

机构地区：[1]武夷学院生态与资源工程学院,福建武夷山354300

出　　处：《武夷学院学报》2017年第3期1-5,共5页Journal of Wuyi University

基　　金：福建省科技创新平台建设计划项目(2013N2009);福建省科技厅战略性新兴产业重点项目(2014N0030);国家级大学生创新创业训练计划(201410397006;201610397006)

摘　　要：为探索香水百合试管鳞茎诱导与增殖的适宜培养条件,以其鳞片为外植体,采用正交试验设计,研究了蔗糖、噻苯隆(TDZ)、烯效唑(S_(3307))、二氯苯氧乙酸(2,4-D)等因素对试管小鳞茎形成的影响,结果表明:诱导香水百合试管小鳞茎形成的最优培养基是MS+1.5 mg/L TDZ+2 mg/L S_(3307)+0.2 mg/L 2,4-D+60g/L蔗糖;添加一定浓度的PP_(333)可以促进小鳞茎的增殖生长,适宜试管鳞茎增殖的培养基为MS+10 mg/L PP_(333)。To find out optimizing culture conditions for test-bulb formation and propagation of Lilium casablanca,the effects of TDZ.S3307,2,4-D and sucrose in different concentration on the test-tube bulb induction and formation were studied with orthogonal test design,taking lily scales as explants.The results showed that the optimal culture medium for bulblet induction was MS＋1.5 mg/L TDZ ＋ 2 mg/L S3307＋ 0.2 mg/L 2,4-D ＋60 g/L sugar.A certain concentration of PP333 added in MS medium promoted the growth and proliferation of lily bulblets,and the optimal medium for bulblet propagation was MS＋ 10 mg/L PP333.

关 键 词：香水百合 鳞茎 组织培养 正交试验设计 

分 类 号：S723.132[农业科学—林木遗传育种]