苦豆子浸提物对伽师瓜蔗糖合成酶和酸性转化酶活性的影响  

Effects of Solvent Extracts From Sophoraa lopecuroides L. on Sucrose Synthase and Acid Invertase Activities of Jiashi Melon

作　　者：党艳青[1] 刘琛璠 叶林[2,3] DANG Yanqing LIU Chenfan YE Lin(College of Plant Sciences,Tarim University, Alar,Xinjiang 843300 College of Life Sciences,Tarim University, Alar, Xinjiang 843300 Construction Corps Key Laboratory of Special Agricultural Products Further Processing in Southern Xinjiang,Alar, Xinjiang 843300)

机构地区：[1]塔里木大学植物科学学院,新疆阿拉尔843300 [2]塔里木大学生命科学学院,新疆阿拉尔843300 [3]南疆特色农产品深加工兵团重点实验室,新疆阿拉尔843300

出　　处：《北方园艺》2017年第9期24-29,共6页Northern Horticulture

基　　金：南疆特色农产品深加工兵团重点实验室资助项目(AP1502)

摘　　要：以伽师瓜幼苗为研究对象,分别采用苦豆子的不同溶剂萃取物喷施处理,测定伽师瓜叶片中蔗糖合成酶(SS)和酸性转化酶(AI)活性的变化,以期为甜瓜栽培施用苦豆子绿肥提供科学依据。结果表明:除正丁醇萃取物外,其它3种溶剂萃取物处理对伽师瓜叶片中SS和AI活性均有显著影响(P<0.05),以伽师瓜叶片中的酶净活性(SS-AI)为指标,不同萃取物处理对伽师瓜叶片蔗糖酶净活性的影响大小为二氯甲烷>石油醚>正丁醇>空白对照>乙酸乙酯,其中,二氯甲烷处理对伽师瓜叶片中SS和AI活性影响达到极显著水平(P<0.01),1.5g·L^(-1)处理组的SS活性在20d达到4.56 mg·h^(-1)·g^(-1),较空白处理组(2.10 mg·h^(-1)·g^(-1))提高了117.14%,而AI活性仅为0.71mg·h^(-1)·g^(-1),较空白对照组(1.04mg·h^(-1)·g^(-1))降低了31.73%,说明苦豆子浸提物喷施处理能够促进伽师瓜中蔗糖合成与积累。The seedlings of Jiashi melon were taken as experiment materials.After spraying extracts fromSophora alopecuroides L.on Jiashi melon seedlings,the activity of sucrose synthase and acid invertase of Jiashi melon leaves were determined.The results showed that all the extracts,except for n-butanol extract,could obviously affect the SS and AI activities（P〈0.05）.The net enzyme activity（SS-AI）was taken as reference index,and the effect of the net enzyme activity among all treatment groups were as follow,dichloromethane,petroleum ether,nbutanol,blank control,ethyl acetate.Moreover,the effects of SS and AI activities reached extremely significant difference by spraying dichloromethane extracts（P〈0.01）.The SS activity of treatment group,1.5g·L-1dichloromethane extracts,reached the highest 4.56mg·h-1·g-1 at the 20 days.Comparing with the blank group（2.10mg·h-1·g-1）,the activity of SS was promoted 117.14%.Meanwhile,the activity of AI was just0.71mg·h-1·g-1.Comparing with the blank group（1.04mg·h-1·g-1）,the activity of AI was lowered31.73%.In all,these results demonstrated that some compounds of the extracts fromSophora alopecuroides L.could enhance the sucrose synthesis and accumulation of Jiashi melon.

关 键 词：苦豆子 蔗糖合成酶 酸性转化酶 伽师瓜 

分 类 号：Q946[生物学—植物学]