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作 者:李艳青[1] 赵玉翠[2] 潘好芹[1] LI Yanqing ZHAO Yucui PAN Haoqin(Biological Engineering Research and Development Center, Weifang University of Science and Techology, Shouguang, Shandong 262700 College of Applied Chemistry and Biological Technology ,Shandong Normal University-Lishan College,Qingzhou, Shandong 262500)
机构地区:[1]潍坊科技学院生物工程研发中心,山东寿光262700 [2]山东师范大学历山学院生物与化学学院,山东青州262500
出 处:《北方园艺》2017年第9期106-110,共5页Northern Horticulture
基 金:潍坊市科学技术发展计划资助项目(2014GX048)
摘 要:以携带褐纹病菌的茄子叶片为试材,采用室内分离鉴定、致病性测定、室内抑菌试验等方法进行病原分离,并研究了病原菌的致病性及抗药性,为田间茄子褐纹病的诊断与防治提供参考依据。结果表明:共分离到9个菌株,菌株类型有产生α型分生孢子、β型分生孢子、混合型分生孢子及只产生菌丝不产生载孢体类型;致病性测定发现菌株PvTF-11、PvVSJ-22、PvVL-211对茄子叶片致病性较强;茄子褐纹病菌的室内抑菌试验发现PvVL-621、PvVL-211属于药剂敏感型菌株,PvVSJ-101、PvVSJ-102属于抗药型菌株。说明在人工培养条件下茄子褐纹病菌也可以产生β型分生孢子及混合型分生孢子类型;且茄子褐纹病菌已产生抗药性菌株。Leaves of eggplant with Phomopsis rot bacteria were used as materials.Via indoor separation identification,determination of pathogenic and bacteriostatic test methods,the pathogenic bacteria were isolated and the pathogenicity and resistance to drugs were studied.The results showed that nine strains were obtained,which containing generating α-conidia,β-conidia,hybrid conidia and only making mycelium type.PvTF-11,PvVSJ-22and PvVL-211 had stronger pathogenicity for eggplant leaf by determination of pathogenic.Antibacterial experiment found that PvVL-621,PvVL-211 belonged to the drug sensitive strains,PvVSJ-101,PvVSJ-102 type belonged to the resistant strains.So eggplant Phomopsis rot bacteria could also produceβ-conidia and hybrid conidiophore type under the condition of artificial culture.And eggplant Phomopsis rot bacteria had resistant strains.
分 类 号:S436.411[农业科学—农业昆虫与害虫防治]
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