肝纤维化大鼠肝气郁结证与肝藏血关系的研究  

Study the relationship between liver qi stagnation and liver stores blood in liver fibrosis rats

作　　者：阎晶璐[1] 薛晓兴 李君玲[1] 夏恺[1] 王田[1] 王伟[1] YAN Jing-lu XUE Xiao-xing WANG Wei et al(Beijing university of traditional chinese medicine （ Beijing, 100029） China)

机构地区：[1]北京中医药大学研究院,北京100029

出　　处：《中西医结合肝病杂志》2017年第2期97-100,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家"十二五"973项目(No.2011CB505106)

摘　　要：目的:探讨中医藏象理论"肝藏血主疏泄"相关肝脏病理生理学基础。方法:以四氯化碳皮下注射法建立肝纤维化肝气郁结证大鼠模型,利用血生化检测法及高效液相色谱检测法,研究其血清及脑部主要蛋白标志物的含量变化。结果:在肝纤维化病程之中大鼠的肝纤维化标志物ALT、AST、Ⅳ-C、LV、HA、TGF-b,脑血管轴标志物ANG-Ⅱ、NE,脑肠轴标志物5-HT、CRH-ACTHCORT等均有升高,神经内分泌轴标志物等多种蛋白含量均有发生变化。结论:血清及脑部主要蛋白标志物含量变化与肝藏血主疏泄理论具有相关性。Objective：To explore the biological mechanism of ＂liver storing blood and Liver Controlling Disper＂. Methods：The rat liver fibrosis model was established by subcutaneous injection of carbon tetrachloride;The functional magnetic resonance imaging and liver uhra- sound were used to detect brain and liver blood flow velocity, the vein diameter and blood flow in the model rats. Results：The test result shows that vein diameter and blood flow of the hepatic artery and hepatic vein, the blood flow of hippocampus and hypothalamus were in- creased in liver fibrosis. Also abnormal activation of brain amygdala was found in brain functional magnetic analysis. Conclusion ：The results were proved to relate to ＂liver storing blood ＂theory closely. The abnormal function of ＂liver storing blood＂ was closely related to the hemody- namics changes of liver and brain.

关 键 词：肝纤维化大鼠 肝气郁结证 肝藏血 血清蛋白标志物 

分 类 号：R259[医药卫生—中西医结合]