丹参酮胶囊对大鼠同种异体肝移植后缺血再灌注肝损伤的防护作用  

The protective effect of Salvia miltiorrhiza Capsule on ischemia reperfusion injury after liver transplantation in rats

作　　者：秦雪琴[1] 徐航[1] 吴文琴[2] 雷尚芳 陈悦[1] QIN Xue-qin XU Hang WU Wen-qin etal(Department of infectious disease, Shiyan Taihe Hospital （Affiliated Hospital of Hubei Medi- cal College） （ Shiyan Hubei, 442000） China.)

机构地区：[1]湖北省十堰市太和医院(湖北医药学院附属医院)感染科,湖北十堰442000 [2]十堰市太和医院急诊科

出　　处：《中西医结合肝病杂志》2017年第2期101-103,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省卫生和计划生育委员会西医类重点项目(No.WJ2015MB224)

摘　　要：目的:研究丹参酮胶囊对大鼠同种异体肝脏移植后P_(450)亚型酶及肝功能的影响,分析术后缺血再灌注肝损伤的防护机制。方法:SD大鼠64只,随机分为对照组、模型组、丹参酮胶囊小、大剂量组,除对照组外其余3组大鼠均采用改良双袖套法建立大鼠同种异体肝移植模型,丹参酮胶囊小、大剂量组大鼠分别用1%和2%丹参酮胶囊溶液灌胃3W,对照组及模型组大鼠灌等体积生理盐水。3 W后取静脉血检测大鼠肝功能五项、血清总超氧化物歧化酶(T-SOD)、白细胞介素-6(IL-6)、前列腺素E2(PGE2)水平;取大鼠肝脏检测肝微粒体P_(450)亚型酶活性。结果:丹参酮胶囊小、大剂量组大鼠T-SOD明显提高、CYP2E1、CYP2C9酶活性、IL-6、PGE2及r-GT、TBA、TBil明显下降,存活率分别达56.25%及62.5%,与模型组比较,P<0.05,差异有统计学意义。结论:丹参酮胶囊可提高同种异体大鼠肝脏移植术后T-SOD活性,促进氧自由基清除酶清除氧自由基、降低炎症因子IL-6、PGE2刺激机体造成的炎症反应,并通过抑制P_(450)亚型酶活性来减轻药物代谢时对肝脏的毒性,其活血化瘀的药理特性可增加移植肝脏组织供氧,促进肝功能恢复,提高肝脏移植存活率。Objective：To study the effects of Salvia miltiorrhiza Capsule on ischemia reperfusion injury after liver transplantation in rats and to analyze its protective mechanism. Methods ：64 male SD rats were randomly divided into control group, model group, tanshinone cap- sule low and high dose group, except the control group were treated with modified double cuff method to establish rat orthotopic liver trans- plantation model, tanshinone capsule low and high dose group were used 1% and 2% of tanshinone capsule solution by gavage for 3 weeks （W）, control group and model group were given equal volume of saline. After 3 W, liver function, serum total superoxide dismutase （T- SOD）, interleukin-6 （IL-6）, prostaglandin E2 （PGE2） level and liver microsomal P450 subunit activity were detected in the rat liver. Re- suits：compared with the model group, The T-SOD in tanshinone capsule low, high dose group increased significantly;CYP2E1, CYP2C9 en- zyme activity, IL-6, PGE2, GGT, total bile acid （TBA）, STB decreased significantly, the survival rate were 56.25% and 62.5%, respec- tively, the difference was statistically significant （ P 〈 0.05 ）. Conclusion ： Tanshinone capsule could increase the T-SOD activity after al- lograft orthotopic liver transplantation in rats, promote oxygen free radical scavenging enzyme oxygen free radical removal, reduce inflammato- ry cytokines IL-6 and PGE2 stimulated body.

关 键 词：丹参酮胶囊 同种异体肝移植 R450亚型酶 肝功能 防护作用 

分 类 号：R657.3[医药卫生—外科学]