De novo DNA methylation during monkey pre-implantation embryogenesis  

作　　者：Fei Gao Yuyu Niu Yi Eve Sun Hanhn Lu Yongchang Chen Siguang Li Yu Kang Yuping Luo Chenyang Ni Juehua Yu Chang Li Nianqin Sun Wei Si Hong Wang Weizhi Ji Tao Tan 

机构地区：[1]Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China [2]Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120, China [3]National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan 650500, China [4]Translational Stem Cell Research Center, Tong/i Hospital Tong/i University School of Medicine, Shanghai 200092, China [5]Department of Psychiatry and Biobehavioral Sciences, UCLA Medical School, Los Angeles, CA 90095, USA

出　　处：《Cell Research》2017年第4期526-539,共14页细胞研究（英文版）

基　　金：This work was supported by the Major State Basic Research Development Program of China （973 Program） （2012CBA01300）, the National High Technology Research and Development Pro- gram of China （863 Program） （2012AA020701）, the National Natural Science Foundation of China （31371456, U1302227, 91319309, 31271371, and 31460313）, Yunnan Innovation Talents of Science and Technology （2012HA013）, the National Basic Research Program of China （973 Program） （2010CB126401）, and Agricultural Science and Technology Innovation Program （ASTIP）.

摘　　要：Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report ge- nome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylation is also initiated in the same period. By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methyla- tion. Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. Unlike in rodents, X chromosome inactivation is not observed during monkey pre-implantation development. Our study provides the first comprehensive illustration of the ＇wax and wane＇ phases of DNA methylation dynamics. Most importantly, our DNA methyitransferase loss-of- function analysis indicates that DNA methylation influences early monkey embryogenesis.

关 键 词：rhesus monkey pre-implantation embryogenesis de novo DNA methylation 

分 类 号：Q75[生物学—分子生物学] S814.8[农业科学—畜牧学]