Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system  

作　　者：Ming Ma Fengfeng Zhuang Xiongbing Hu Bolun Wang Xian-Zi Wen Jia-Fu Ji Jianzhong Jeff Xi 

机构地区：[1]Department of Biomedical Engineering, State Key Laboratory of Natural and Biomimetic Drugs, College of Engineering, Peking University, Beijing, China [2]Beijing Viewsolid Biotech Co. Ltd, Beijing 100071, China [3]Division of Gastrointestinal Cancer Translational Research Laboratory, Department of Gastrointestinal Surgery, Key Laboratory of Carcinogene- sis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Fu-Cheng Road, Beijing, China [4]State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Peking University, Beijing 100871, China [5]Collabo- rative Innovation Center for Cardiovascular Disorders, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China

出　　处：《Cell Research》2017年第4期578-581,共4页细胞研究（英文版）

基　　金：This research was supported by National Natural Science Foundation of China （NSFC; 81325010, 81421004 and 31371443） and Special Funding Support of Beijing Municipal Administration of Hospitals, Clinical Medicine Development （ZYLX201701）.

摘　　要：The clustered, regularly interspaced, short palin- dromic repeats （CRISPR）/CRISPR-associated protein 9 （Casg） system is a versatile tool for genomic engineering in mammalian cells and organisms, enabling the intro- duction of site-specific genomic double-strand breaks （DSBs） [1]. The resulting DSBs are repaired by at least two distinct and competitive mechanisms, nonhomolo- gous end-joining （NHEJ） and homology-directed repair （HDR）. The former results in insertions and deletions （indels）, whereas the latter leads to precise genetic modi- fication.

分 类 号：Q[生物学]