星点设计-效应面法优化镰形棘豆总黄酮的提取工艺  

Extraction Optimization of Total Flavonoids from Oxytropis Falcata Bunge by Central Composite Design-response Surface Methodology

作　　者：曾棋平 王艺红[1] 蔡小辉[1] 杨丽娜[1] 陈锦珊[1] 

机构地区：[1]解放军第175医院/厦门大学附属东南医院制剂科,福建漳州363000

出　　处：《中国药师》2017年第5期801-804,共4页China Pharmacist

基　　金：原南京军区医学科技创新项目(编号:14MS085)

摘　　要：目的:优选镰形棘豆总黄酮的提取工艺。方法:以总黄酮含量为指标,在单因素试验的基础上,采用星点设计-效应面法考察溶剂用量、乙醇体积分数、提取时间对镰形棘豆总黄酮含量的影响。结果:最佳提取工艺为加入20倍量66%乙醇回流提取2次,每次84 min,在此条件下,总黄酮的含量达23.21 mg·g^(-1)。结论:采用星点设计-效应面法优选的提取工艺条件稳定可行,预测性好。Objective： To optimize the extraction process of total flavonoids from Oxytropis falcata bunge. Methods： With the content of total flavonoids as the index, and based on the results of single factor tests, central composite design-response surface methodology was adopted to optimize the extraction technology with the solvent amount, ethanol concentration and extraction time as the factors. Results： The optimal extraction conditions were as follows： extracted twice with 20-fold amount of 66% ethanol, and 84 minute per time. Under the above conditions, the extraction rate of total flavonoids was up to 23.21 mg·g^-1. Conclusion： The central composite design-response surface methodology is convenient and highly predictive for the extraction optimization of total flavonoids from oxytropis falcata bunge.

关 键 词：镰形棘豆 总黄酮 星点设计-效应面法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]