多波长HPLC法同时测定皮肤康洗液中5种有效成分的含量  

Simultaneous Determination of Five Active Components in Pifukang Lotion by HPLC with Multiple UV Wavelengths

作　　者：马娜 范桂强 曹毅[2] 齐善厚[3] 

机构地区：[1]衡水市食品药品检验检测中心,河北衡水053000 [2]衡水市第四人民医院 [3]衡水学院

出　　处：《中国药师》2017年第5期963-965,共3页China Pharmacist

摘　　要：目的:建立多波长切换高效液相色谱法同时测定皮肤康洗液中芍药苷、甘草苷、甘草酸铵、大黄素和蛇床子素5种有效成分的含量。方法:色谱柱为Diamonsil C18柱(200 mm×4.6 mm,5μm);流动相为乙腈-0.1%盐酸水溶液,梯度洗脱;流速为1.5 ml·min^(-1);柱温为30℃;检测波长为232 nm(0~6 min,检测芍药苷)、277 nm(6~10 min,检测甘草苷)、254 nm(10 min以后,检测甘草酸铵、大黄素、蛇床子素)。结果:芍药苷、甘草苷、甘草酸铵、大黄素和蛇床子素的线性范围分别为28.20~282.0μg·ml^(-1)(r=0.999 7)、12.150~121.500μg·ml^(-1)(r=0.998 8)、13.420~134.200μg·ml^(-1)(r=0.999 5)、0.047~0.466μg·ml^(-1)(r=0.999 9)、2.380~23.800μg·ml^(-1)(r=0.999 9),平均加样回收率分别为98.49%,99.00%,98.38%,97.36%,97.70%,RSD分别为0.71%,0.62%,0.85%,0.92%,0.78%(n=6)。结论:该方法简便、准确、灵敏度高、重复性好,可用于同时测定皮肤康洗液中上述5个有效成分的含量。Objective： To establish an HPLC method for the simultaneous determination of five active components （paeoniflorin, liquiritin, ammonium glycyrrhizinate, edomin and osthole）in Pifukang lotion with multiple UV wavelengths. Methods： A Diamonsil C ls （200 mm × 4.6 mm,5 μm） column was used with the mobile phase consisting of 0.1% hydrochloric acid and acetonitrile with gradient elution. The flow rate was 1.5 ml·min^-1 , and the column temperature was 30℃. Paeoniflorin was detected at 232 nm（0-6 min）, liquiritin was detected at 277 nm （6-10 min）, and ammonium glycyrrhizinate, edomin and osthole were detected at 254 nm （after 10 min）. Results： The linear range of paeoniflorin, liquiritin, ammonium glycyrrhizinate, edomin and osthole was 28. 200-282. 000μg·ml^-1 （r = 0.999 7）, 12. 150-121. 500μg·ml^-1 （ r = 0.998 8 ）, 13. 420-134.200 μg·ml^-1（ r = 0.999 5 ）, 0. 047-0. 466μg·ml^-1 （ r = 0.999 9） and 2.38-23.8μg·ml^-1 （ r = 0.999 9 ）, respectively. The average recovery （RSD） was 98.49% （0.71%）, 99.00% （0.62%）, 98.38% （0.85%） ,97.36% （0.92%） and 97.70% （0.78%）, respectively （n = 6）. Conclusion： The established method is simple, accurate, highly sensitive and well reproducible, which can be used for the determination of the five active ingredients in Pifukang lotion.

关 键 词：高效液相色谱法 皮肤康洗液 芍药苷 甘草苷 甘草酸铵 大黄素 蛇床子素 含量测定 

分 类 号：R927.2[医药卫生—药学]