Electrochemiluminescence of metal-organic complex nanowires based on graphene-Nafion modified electrode for biosensing application  

作　　者：Qing Li Caihong Kang Kunjie Li Aibing Chen Wei Zhang Yong-Sheng Zhao 

机构地区：[1]College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China [2]Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China [3]Hebei Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science and Technology, Shijiazhuang 050018, China

出　　处：《Science China Chemistry》2017年第5期642-648,共7页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21406053,21521062);the Ministry of Science and Technology of China(2012YQ120060);Natural Science Foundation of Hebei Province(B2016208082);Science and Technology Research Projects in Hebei Universities(QN2015243,YQ2014015);Five Platform Open Fund Projects of Hebei University of Science and Technology(2014YY25)

摘　　要：In this work, we chose tris（2,2＇-bipyridyl）ruthenium（II）hexafluorophosphate （Ru（bpy）3（PF6）2）, a metal-organic complex material, to prepare nanowires, which were subsequently applied for the construction of electrochemiluminescence （ECL） biosensor by immobilizing them onto a glassy carbon electrode （GCE） with graphene-Nafion composite films. The graphene therein, being a two-dimensional carbon nanomaterial with outstanding electronic properties, can obviously improve the conductivity of the Nation film, as well as enhance the electrochemical signal and ECL intensity of the Ru（bpy）3（PF6）2 nanowires （RuNWs） at low graphene concentration. The developed biosensor exhibited excellent ECL stability with tripropylamine （TPrA） as co-reactant. The ECL biosensor exhibited high sensitive ECL response in a wide linear range and low detection limit for the detection of proline. It is considered that the oxidation products of proline would be responsible for the ECL enhancement. The large electro-active area of the nanowires and the enhancement effect of the graphene played critical roles in the high detection performance of the ECL biosensor. The results demonstrated herein may provide a useful enlightenment for the design of more sensitive ECL biosensors.In this work, we chose tris(2,2′-bipyridyl)ruthenium(II)hexafluorophosphate(Ru(bpy)3(PF6)2), a metal-organic complex material,to prepare nanowires, which were subsequently applied for the construction of electrochemiluminescence(ECL) biosensor by immobilizing them onto a glassy carbon electrode(GCE) with graphene-Nafion composite films. The graphene therein, being a two-dimensional carbon nanomaterial with outstanding electronic properties, can obviously improve the conductivity of the Nafion film, as well as enhance the electrochemical signal and ECL intensity of the Ru(bpy)3(PF6)2 nanowires(RuNWs) at low graphene concentration. The developed biosensor exhibited excellent ECL stability with tripropylamine(TPrA) as co-reactant. The ECL biosensor exhibited high sensitive ECL response in a wide linear range and low detection limit for the detection of proline. It is considered that the oxidation products of proline would be responsible for the ECL enhancement. The large electro-active area of the nanowires and the enhancement effect of the graphene played critical roles in the high detection performance of the ECL biosensor. The results demonstrated herein may provide a useful enlightenment for the design of more sensitive ECL biosensors.

关 键 词：metal-organic nanowires electrochemilumincscence GRAPHENE bioscnsor 

分 类 号：O657.1[理学—分析化学]