A highly sensitive DNA-AIEgen-based ＂turn-on＂ fluorescence chemosensor for amplification analysis of Hg^2+ ions in real samples and living cells  

作　　者：Xiaowen OU Xiaoding Lou Fan Xia 

机构地区：[1]Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China [2]Shenzhen Institute of Huazhong University of Science and Technology, Shenzhen 518000, China

出　　处：《Science China Chemistry》2017年第5期663-669,共7页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21525523,21574048,21375042,21405054);the National Basic Research Program of China(2015CB932600,2013CB933000);the Special Fund for Strategic New Indus-try Development of Shenzhen,China(JCYJ20150616144425376);1000 Young Talent(to Fan Xia)

摘　　要：Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled(fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids(AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease(DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg^(2+) are achieved. This robust assay allows for sensitive and selective detection of Hg^(2+) in real water samples and mapping of intracellular Hg^(2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.Functional nucleic acids （FNAs）-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled （fluorophore and quencher group） DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids （AFNAs） probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease （DSN） enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg2＋are achieved. This robust assay allows for sensitive and selective detection of Hg2＋ in real water samples and mapping of intracellular Hg2＋, without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.

关 键 词：functional nucleic acids AIEgen Hg2+ detection real samples living cells 

分 类 号：O657.3[理学—分析化学] TP212.2[理学—化学]