一株伏马毒素降解菌的筛选与鉴定  

Screening and Identification of A Fumonisins Degradation Bacteria

作　　者：杨朋飞[1,2] 常晓娇[2,3] 伍松陵[2] 王峻[2] 王楠希 屈凌波[1] 孙长坡[2] Yang Pengfei Chang Xiaojiao Wu Songling Wang Jun Wang Nanxi Qu Lingbo Sun Changpo(College of Bioengineering, Henan University of Technology, Zhengzhou 450001 Academy of State Administration of Grain, Beijing 100037 College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001)

机构地区：[1]河南工业大学生物工程学院,郑州450001 [2]国家粮食局科学研究院,北京100037 [3]河南工业大学粮油食品学院,郑州450001

出　　处：《中国粮油学报》2017年第4期110-115,共6页Journal of the Chinese Cereals and Oils Association

基　　金：国家重点基础研究发展计划(2013CB127805)

摘　　要：以伏马毒素FB_1为唯一碳源,采用富集培养法从污泥样品中分离到1株高效降解FB_1的菌株。经高效液相色谱(HPLC)法检测,该菌在液体无机盐培养基(MSM)中对FB_1有良好的削减能力,5 d内可将25μg的FB_1完全降解。通过对该菌的16S rDNA序列进行同源性分析,并结合生理生化试验,最终鉴定为鞘氨醇盒菌(Sphingopyxis sp.),命名为ASAG22。ASAG22细胞内产生的活性物质将FB_1降解为4种质荷比分别为406.352 7、288.209 9、264.182 7和220.156 9的新物质,且该活性物质经蛋白酶K处理而失去活性。Using fumonisins FB1 as the sole carbon source, a bacterial strain efficiently degrading the FBI deg- radation strain was screened from sludge samples by an enrichment culture approach. The strain, finally named ASAG22, can degrade FB1 well in liquid MSM culture and completely degrade 25 μg FB1 in 5 days by high perform- ance liquid chromatography detection. It was identified as Sphingopyxis sp. on the basis of homology analysis of the 16S rDNA sequence analysis combining with physiological and biochemical test. Further research about the degrada- tion mechanism of strain showed that the active substance generated from ASAG22 cell degraded FB1 into four new degradation products, i. e. , 406. 352 7, 288. 209 9, 264. 182 7 and 220. 156 9. And the active material can inacti- vate through the treatment of proteinase K.

关 键 词：伏马毒素 鞘氨醇盒菌 生物降解 

分 类 号：Q939.9[生物学—微生物学]