RNA-seq reveals mechanisms of SlMX1 for enhanced carotenoids and terpenoids accumulation along with stress resistance in tomato  

作　　者：Mohamed Ewas Yangqiang Gao Farhan Ali Elsayed M. Nishawy Raheel Shahzad Hizar Subthain Mohamed Amar Cathie Martin Jie Luo 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), College of Life Sdence and Technology, Huazhong Agricultural University, Wuhan 430070, China [2]Egyptian Deserts Gene Bank (EDGB), Genetic Resources Department Cairo, Egypt [3]Deserts Research Center (DRC), Cairo, Egypt [4]John lnnes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK

出　　处：《Science Bulletin》2017年第7期476-485,共10页科学通报（英文版）

基　　金：supported by the Major State Basic Research Development Program of China(2011CB100601);the National Natural Science Foundation of China(31070267);the Program for New Century Excellent Talents in University of Ministry of Education in China(NCET-09-0401)

摘　　要：Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human civilization.Our previous study reported that manipulation of Sl MX1 gene enhanced carotenoids accumulation and drought resistance in tomato.Here,RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of Sl MX1 involved in stress resistance and enhanced secondary metabolites.Physiological analysis showed that overexpression of Sl MX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic(fungus,bacteria,virus and insects) stresses in tomato.This research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome density.In addition,we reported two types of trichome which seems to be aberrant types in tomato.This study unravels the mechanism of regulation of Sl MX1,which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human civilization. Our previous study reported that manipulation of SlMX1 gene enhanced carotenoids accumulation and drought resistance in tomato. Here, RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of SlMX1 involved in stress resistance and enhanced secondary metabolites. Physiological analysis showed that over- expression of SlMX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic （fungus, bacteria, virus and insects） stresses in tomato. This research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome density. In addition, we reported two types of trichome which seems to be aberrant types in tomato. This study unravels the mechanism of regulation of SlMX1, which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.

关 键 词：TOMATO SlMX1 Resistance Carotenoids TERPENOIDS Mechanism RNA Seq 

分 类 号：S641.2[农业科学—蔬菜学]