HPLC同时测定藤珠胃康颗粒中4个皂苷类成分  

Simultaneous Determination of Four Saponins in Tengzhuweikang Granules by HPLC

作　　者：王小平[1] 王芳[1] 白吉庆[1] 王金[1] 叶峥嵘[1] 权利娜[1] 沈舒文[1] 

机构地区：[1]陕西中医药大学,陕西咸阳712046

出　　处：《中国现代应用药学》2017年第4期553-556,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：陕西省科技厅中药现代化项目(2012KTCL03-14);陕西省科学技术研究发展计划项目[2009K19-03(2)]

摘　　要：目的建立HPLC同时测定藤珠胃康颗粒中4个皂苷类成分(人参皂苷Re、人参皂苷Rb_1、人参皂苷Ro和竹节参皂苷Ⅳa)的方法。方法采用Hypersil GOLD C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%磷酸水溶液,梯度洗脱(-5~0 min,19%乙腈;0~14 min,19%→26%乙腈;14~22 min,26%→29%乙腈;22~30 min,29%乙腈;30~40 min,29%→35%乙腈;40~55 min,35%乙腈),体积流量1.0 mL·min^(-1),检测波长203 nm,柱温30℃。结果人参皂苷Re在0.080 4~1.608μg(r=1),人参皂苷Rb_1在0.108 8~2.176μg(r=0.999 9),人参皂苷Ro在0.288 8~5.776μg(r=0.999 9),竹节参皂苷Ⅳa在0.176 0~3.520μg(r=0.999 9)内线性关系良好;平均回收率(n=6)分别为99.41%,101.92%,99.76%,100.31%,RSD值分别为2.22%,2.07%,0.33%,0.64%。结论本实验所建立的方法简单,专属性强,重复性好,可用于藤珠胃康颗粒中人参皂苷Re、人参皂苷Rb_1、人参皂苷Ro和竹节参皂苷Ⅳa的测定。OBJECTIVE To establish a method for simultaneous determination of four saponins（ginsenoside Re, ginsenoside Rbl, ginsenoside Ro and chikusetsusaponin IVa） in Tengzhuweikang granules by HPLC. METHODS The HPLC separation was achieved on a Hypersil GOLD C18（250 mm×4.6 mm, 5 μm） column. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution with gradient elution（-5-0 min, 19% acetonitrile; 0-14 rain, 19%426% acetonitrile; 14-22 min, 26%-29% acetonitrile; 22-30 min, 29% acetonitrile; 30-40 min, 29%-35% aeetonitrile; 40-55 min, 35% acetonitrile） at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 203 nm and the colum temperature was 30 ℃. RESULTS The linear ranges of ginsenoside Re, ginsenoside Rbl, ginsenosideRo, chikusetsusaponin IVa were 0.080 4-1.608 μg （r=1）, 0.108 8-2.176 μg（r=0.999 9）, 0.288 8-5.776 μg（r=0.999 9）, 0.176 0-3.520 μg（r=0.999 9）, respectively. The average recoveris（n=6） were 99.41%, 101.92%, 99.76%, 100.31%, respectively. RSD of the recoveris was 2.22%, 2.07%, 0.33%, 0.64%, respectively. CONCLUSION The method is simple and repeatable, can be used for the determination of ginsenoside Re, ginsenoside Rbb ginsenoside Ro and chikusetsusaponin IVa in Tengzhuweikang Granules.

关 键 词：藤珠胃康颗粒 人参皂苷RE 人参皂苷RB1 人参皂苷Ro 竹节参皂苷Ⅳa 

分 类 号：R917[医药卫生—药物分析学]