应用高通量测序技术对进境澳大利亚小麦病原真菌的检测研究  

Application of high-throughput sequencing technology in the detection of pathogenic fungi in imported Australian wheat

作　　者：史亚千[1] 程颖慧[1,2,3] 王颖[1,2] 高瑞芳[1,2] 汪莹[1,2] 向才玉[1,2] 冯建军[1,2] 娄定风[1,2] 王红英[1,2] 朱崧琪[1] 

机构地区：[1]深圳出入境检验检疫局,广东深圳518045 [2]深圳市检验检疫科学研究院深圳外来有害生物检测技术研发重点实验室 [3]昆明理工大学

出　　处：《植物检疫》2017年第1期26-31,共6页Plant Quarantine

基　　金：国家质量监督检验检疫总局科技计划项目(2015IK267);深圳出入境检验检疫局科技计划项目(SZ2015219)

摘　　要：相比较其他检测技术,高通量测序技术具有高效、快速等特点,并拥有全面分析复杂核苷酸群体的功能,本文运用高通量测序技术对进境澳大利亚4批次小麦种子和筛下物上的病原真菌进行检测,通过基因组DNA提取、高通量测序、生物信息学分析后得到分类OTU共622个,基于不同分类水平,分属于5个门、64个目、201个属。检测显示,种子与筛下物携带的病原真菌的种类和比例有较大差异,并且筛下物携带的病原真菌数量和种类远多于种子携带的病原真菌。Compared with other detection technologies,high-throughput sequencing technology is efficient, rapid,and has a comprehensive analysis of the function of complex nucleotide groups,In this paper, high-throughput sequencing technology was applied to detect the pathogenic fungi in wheat seeds and sieves from 4 batches of wheat in Australia. A total of 622 OTUs were obtained by genomic DNA ex- traction,high-throughput sequencing and bioinformatics analysis,and belonged to 5 Phylums,64 orders and 201 Genuses based on different taxonomic levels. The results showed that there were significant differ- ences in the types and proportions of the pathogenic fungi carried by the seeds and sieves,and the num- ber and type of pathogenic fungi carried by the sieve were much more than the pathogenic fungi carried by the seeds.

关 键 词：高通量测序 小麦病原真菌 检测 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]