绒毛细胞培养方法的比较及绒毛细胞核型分析在胚胎停育中的应用  

The comparison of two culture methods and karyotype analysis for villus cells in the embryo damage

作　　者：汤素环[1] 李付广[1] 谭卫荷[1] 唐江[1] 郭晓燕[1] TANG Su-huan LI Fu-guang TAN Wei-he TANG Jiang GUO Xiao-yan(Guangzhou Medical University Sixth Affiliated Hospital, Prenatal Diagnosis Center, Qingyuan 511518, China)

机构地区：[1]广州医科大学附属第六医院产前诊断中心清远市人民医院,511518

出　　处：《中国优生与遗传杂志》2017年第4期63-64,133,共3页Chinese Journal of Birth Health & Heredity

基　　金：清远市科技计划项目(00161751320722022)

摘　　要：目的探讨两种绒毛细胞培养方法对绒毛细胞培养结果的影响以及其染色体核型分析在胚胎停育中的应用。方法取103例胚胎停育的绒毛细胞分别采用胰酶与胶原酶混合消化法和组织块直接培养法进行细胞培养、染色体核型分析。结果 103例绒毛组织两种方法均培养成功98例,培养成功率为95.1%,胰酶与胶原酶混合消化法比组织块培养法培养时间更短。共分析绒毛标本98例,发现异常核型42例,异常率42.86%,其中数目异常最多(39例)。结论胰酶与胶原酶混合消化法可以缩短绒毛细胞培养时间,绒毛染色体核型异常是胚胎停育的重要因素。Objective：To study the effect of two methods to the result of villus cultivate and discuss the application value of villus cells karyotype in the embryo damage. Methods：Villus cells from 103 women of the embryo damage were cultured for two methods：tissue block culture and cells cultured after Trypsin and collagenase digestion and prepared for karyotype analysis. Result：103 cases were cultured and 98 cases for two methods were all cultured successfully with the successful rate of 95.1%. The time of cell culture after rypsin and collagenase digestion was shorter than that of tissue block culture. Analysis of villus cell of 98 cases,42 cases were detected with adnormal karyotype,accounting for 42.86%. And adnormal number was the major type of adnormal karyotype（39 cases）. Conclusion：The time of cell culture after Trypsin and collagenase digestion was shorter than that of tissue block culture.Embryo villus cell abnormal karyotype is one of the important factors of the embryonic damage.

关 键 词：绒毛细胞 组织块培养法 胰酶与胶原酶混合消化法培养 染色体核型分析 胚胎停育 

分 类 号：R714.2[医药卫生—妇产科学]