RNAi载体构建新方法及其在植物次生代谢调控中的应用  

A new method of RNAi vector construction and its application of secondary metabolism regulation of plants

作　　者：曲子越 李影[1] 李林夕 王朝焕 王敏[1] 尹静[1,2] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]东北林业大学林学院林木遗传育种与生物技术国家重点实验室,黑龙江哈尔滨150040

出　　处：《中草药》2017年第7期1458-1465,共8页Chinese Traditional and Herbal Drugs

基　　金：东北林业大学本科创新课题(201510225155);国家自然科学基金资助项目(31570589);中央高校基本科研业务费资助(257-2015DY01)

摘　　要：RNA干扰(RNA interference,RNAi)技术具有专一性强、抑制效率高、可大规模操作、重复性好及在植物中可以孟德尔方式遗传等优点,被广泛应用于基因功能验证、代谢调控等方面。在植物代谢工程中,可通过RNAi方法抑制分支途径产物合成及改变代谢流的分配,调控目标物质的合成。对RNAi技术发生机制、高效RNAi载体构建方法及其在代谢调控中的应用进展进行系统概述,以期为利用RNAi技术进行代谢工程调控研究奠定理论基础和提供技术参考。RNA interference （RNAi） technology has strong specificity, high inhibitory efficiency, large-scale operation application, and good repeatability, and it can perform Mendelian mode of inheritance in plants, which is widely used in the validation of gene function and metabolic regulation. In plant metabolic engineering, the RNAi method can inhibit the synthesis of product, change the distribution of metabolic flux, and regulate the synthesis of the target product. This review summarized the current progress and study strategy of RNAi in plant metabolic regulation. RNAi mechanism and characteristics, optimal strategy of vector structure, efficient RNAi vector construction method, and application progress in the regulation of metabolism were reviewed, in order to lay the theoretical foundation and technical reference of RNAi technology of metabolic engineering control.

关 键 词：RNAI 代谢调控 载体构建 次生代谢 植物代谢工程 

分 类 号：R281[医药卫生—中药学]