环丙沙星体外诱导铜绿假单胞菌对群体感应系统正常与缺陷最低抑菌浓度的影响  

Influence of ciprofloxacin induction in vitro on minimal inhibitory concentrations of Pseudomonas aeruginosa with normal and defective quorum sensing system

作　　者：张智琪[1] 李晓霞[2] 康建邦[2] 高雅婷[1] 张瑞琴[2] 段金菊[2] ZHANG Zhi-qi LI Xiao-xia KANG Jian-bang GAO Ya-ting ZHANG Rui-qin DUAN Jin-ju(Department of Pharmacy, Shanxi Medical University, Shanxi Taiyuan 030001, China Department of Pharmacy, Second Hospital of Shanxi Medical University, Shanxi Taiyuan 030001, China)

机构地区：[1]山西医科大学药学院,山西太原030001 [2]山西医科大学第二医院药学部,山西太原030001

出　　处：《中国医院药学杂志》2017年第8期746-749,共4页Chinese Journal of Hospital Pharmacy

基　　金：山西省卫生计生委科技攻关项目(编号:2014045)

摘　　要：目的:探究群体密度(QS)系统正常与缺陷的铜绿假单胞菌(PA)通过环丙沙星诱导后对其最低抑菌浓度(MIC)影响的区别。方法:将PA按照前期研究的基因测序方法分成正常组和缺陷组。采用琼脂倍比稀释法测定对环丙沙星的MIC值,用1/2MIC、1MIC、2MIC、4MIC的环丙沙星各诱导5代,保存1代、3代和5代的菌株。诱导浓度和诱导代数对MIC值的影响采用重复测量方差分析方法,正常组与缺陷组的比较采用秩和检验。结果:两组组内比较:在同一浓度下,2组各随着诱导代数增加MIC值均增加(P均<0.05);在同一代数下随着诱导浓度的增加,正常组第3代和第5代的MIC值也增加(P均<0.05),缺陷组第3代和第5代的MIC值差异有统计学意义(P均<0.05)。2组组间比较:QS系统分组与诱导代数无交互效应(P>0.05),且缺陷菌株MIC值都低于正常菌株;与诱导浓度存在交互效应(P<0.05)。固定代数固定浓度两组MIC值比较:在1MIC诱导的第3代和第5代,2组之间有统计学差异(P<0.05)。结论:不同诱导浓度对QS系统正常组和缺陷组MIC值的影响有区别。OBJECTIVE To investigate the difference between minimal inhibitory concentrations（MIC）of Pseudomonas aeruginosa（PA）with normal and defective quorum sensing（QS）system induced by ciprofloxacin.METHODS PA was divided into normal group and defective group according to gene sequencing method of the previous studies.Agar dilution method was used to measure MICs of all strains.PA was induced for five generations by ciprofloxacin with four concentrations of 0.5MIC,1MIC,2MIC and4 MIC,generations of the first,third and fifth were saved.Influences of induction times and concentrations on MIC values were analyzed by repeated measures analysis of variance and rank sum test was used to compared normal group and defective group.RESULTS MIC values increased with induction times at the same concentration（P〈0.05）.With induction concentrations under the same times,MIC values of the third and fifth generations in normal group increased（P〈0.05）and defective strains were lower than those of the normal group.MIC value difference between the third and fifth generations in defective group was statistically significant（P〈0.05）.There was crossover effect in groups with induction concentrations（P〈0.05）rather than induction times（P〈0.05）.MIC values in two groups at fixed times and concentrations were significantly different at the third and fifth generations at 1MIC（P〈0.05）.CONCLUSION QS system in normal group and defective group has different influence on MIC values at four concentrations of ciprofloxacin.

关 键 词：铜绿假单胞菌 环丙沙星 群体感应系统 体外诱导 最低抑菌浓度 

分 类 号：R927[医药卫生—药学]