槐果碱对小鼠免疫性肝损伤保护作用的研究  

Study on Protection of Sophicarpine on Immunological Liver Injury in Mice

作　　者：黄银秋[1,2] 周厚琴[1,2] 张璐[1,2] 陆小华[1,2] 魏士长 赵艳玲[2] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]解放军第302医院药学部,北京100039

出　　处：《中国医院用药评价与分析》2017年第3期297-299,303,共4页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：国家自然科学基金资助项目(No.81330090;No.81503350)

摘　　要：目的:探讨槐果碱对polyⅠ:C/D-Gal N诱导的小鼠免疫性肝损伤的干预作用。方法:将C57BL/6小鼠48只随机分为空白组、模型组、槐果碱高剂量组以及槐果碱低剂量组共4组,每组12只。小鼠灌胃给予不同剂量的槐果碱,连续12 d。于末次灌胃后,除空白组外,其余各组小鼠均尾静脉注射polyⅠ:C、腹腔注射D-Gal N造成免疫性肝损伤,12 h后测定小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、胆碱酯酶(CHE)、白蛋白(ALB)以及总蛋白(TP)活性;采用定量即时聚合酶链锁反应测定小鼠肝组织中肿瘤坏死因子α(TNF-α)、γ干扰素(INF-γ)的RNA水平;同时进行肝组织病理学观察。结果:槐果碱能明显降低小鼠ALT、AST以及TNF-α、INF-γ的mRNA水平,抑制CHE、ALB以及TP水平的降低。病例切片显示,与模型组比较,槐果碱可减轻肝组织损伤程度,且呈剂量依赖性。结论:槐果碱对polyⅠ:C/D-Gal N诱导的小鼠免疫性肝损伤具有很好的治疗作用。OBJECTIVE： To probe into the intervention effects of sophicarpine on poly Ⅰ： C/D-Gal N induced immunological liver injury in mice. METHODS： 48 C57BL/6 rice were randomly selected to be divided into four groups： blank group,model group,sophicarpine high dose group and sophicarpine low dose group,12 cases in each.Mice were injected with different doses of sophicarpine by intragastric administration for 12 days. After the last intragastric administration,except for blank group,all rice were injected with polyⅠ： C（ i. v） and D-Ga IN（ i. p） to develop the model of immunological liver injury. The activity of ALT,AST,CHE,ALB and TP after 12 hours and the mRNA level of TNF-α,INF-γ in the liver tissue were detected by Q-PCR. The histological examination of liver tissue were conducted at the same time. RESULTS： After being treated with sophicarpine,the serums level of ALT,AST and mRNA level of TNF-α,INF-γ were obviously decreased and the serum of CHE,ALB and TP were significantly increased. According to pathological section,compared with model group,sophicarpine can reduce the degree of injury and were in a dose-dependent manner. CONCLUSIONS： Sophicarpine has an outstanding prevention treatment of polyⅠ： C/D-Gal N induced immunological liver injury in mice.

关 键 词：槐果碱 polyⅠ:C/D-GalN 免疫性肝损伤 干预作用 

分 类 号：R965[医药卫生—药理学]