P16^(INK4a)和Ki67在宫颈上皮内瘤变中的表达及病理诊断意义  

Expression of P16^(INK4a) and Ki67 in the Cervical Intraepithelial Neoplasia by Immunohistochemistry and Their Significance in Pathological Diagnosis

作　　者：王琳[1] 许发美 周自强[2] 吕秀萍[3] 

机构地区：[1]潍坊医学院妇产科学教研室,山东潍坊261053 [2]淄博市中心医院病理科 [3]潍坊医学院附属医院妇科

出　　处：《潍坊医学院学报》2017年第2期126-128,共3页Acta Academiae Medicinae Weifang

摘　　要：目的探讨P16^(INK4a)和Ki67在宫颈上皮内瘤变(CIN)组织中阳性表达的意义及病理诊断价值。方法选取经病理诊断为CIN的宫颈组织石蜡包埋标本62例,其中CINⅠ级22例,CINⅡ级18例,CINⅢ级22例,采用SP法分别进行P16^(INK4a)和Ki67的免疫组化染色并检测其表达。结果 P16^(INK4a)和Ki67在CINⅠ~Ⅲ级组织中的阳性表达率均逐渐升高,差异均有统计学意义(P<0.01),P16^(INK4a),Ki67的阳性表达率与CIN病变级别呈正相关(r_1=0.658,r_2=0.604,P<0.01)。结论 P16^(INK4a)和Ki67可以作为CIN分级诊断的辅助指标,有利于提高诊断的准确性和一致性,为临床合理治疗提供有效信息。Objective To explore the significance and role about positive expression of P16^INK4a and Ki67 in cervical intraepithelial neoplasia（CIN）.Methods Sixty-two cases of cervical tissues embedded in paraffin that the pathological diagnosis was CIN were selected,which consisted of 22 CINⅠ,18 CINⅡ,22 CINⅢ.Immunohistochemical staining for P16^INK4a and Ki67 were performed on all tissues to detect their expressions.Results The positive expression rates of P16^INK4a,Ki67 were gradually increased with the grading CIN,and expressions of P16^INK4a and ki67 were both positively correlated with the grade of CIN lesion.Conclusion P16^INK4a and Ki67 can be used as auxiliary markers in supporting the diagnosis of grade of CIN lesion.Immunohistochemical staining for P16^INK4a and Ki67 can improve the accuracy and consistency of the diagnosis,and provide effective information for clinical treatment and prognosis.

关 键 词：P16INK4A KI67 宫颈上皮内瘤变 免疫组化 

分 类 号：R711.74[医药卫生—妇产科学]