文心兰OnERS1全长基因克隆及表达分析  

Cloning and Expression Analysis of OnERS1 Gene from Oncidium Gower Ramsey

作　　者：田晓岩[1] 杨翠萍[1] 胡进[1] 闫冰玉 巩笑笑 庄玉粉 潘英文[2] 刘进平[1] 

机构地区：[1]海南省热带生物资源可持续利用重点实验室,海南大学农学院,海口570228 [2]海南出入境检验检疫局热带植物隔离检疫中心,海口570311

出　　处：《分子植物育种》2017年第4期1265-1272,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31560573);海南省科技项目(ZDZX2013023);2015年度海南省普通高等学校研究生创新科研课题(Hys2015-02)共同资助

摘　　要：为了了解乙烯信号受体在文心兰切花衰老中的作用,利用RACE技术克隆了文心兰乙烯受体基因OnERS1(Gen Bank登录号为KP410729)。生物信息学分析表明,OnERS1基因编码631个氨基酸,分子量为70 773.7,等电点为6.82,是跨膜蛋白,与其它植物的ETR乙烯受体蛋白的相似性在74%~99%之间,属ETR1类乙烯受体。基因表达分析结果表明,OnERS1基因在根和蕊柱中表达量最高,在叶片、侧瓣和花萼中次之,在茎和唇瓣中表达量最低;文心兰切花衰老期间OnERS1基因的表达量在第0~6 d持续增加,第6 d后突然下降,第8 d后下降到一个极低的水平。与此同时,花器官中的乙烯释放从第8 d之前缓慢上升,第8~10 d急剧升高。OnERS1基因的时空表达特异性表达说明它可能与花衰老有关。OnERS1（Gen Bank accession number is KP410729）,a member of ethylene receptor gene family was cloned by RACE techniqued from Oncidium Gower Ramsey in order to understand the role of ethylene receptor gene in the senescence of cut flower of Oncidium.Bioinformatics analysis showed that OnERS1 gene a trans-membrane protein encoded 631 amino acids with a molecular weight of 70 773.7,an isoelectric point（IP）of 6.82.OnERS1 shared a similarity of 74%-99% among the ethylene receptor proteins of other plant species and belonged to the ETR1 subfamily.Gene expression analysis demonstrated that OnERS1 gene had the highest expression levels in roots and gynandria,followed by those in sepals,lateral petals and leaves,lowest in stems and lip petals;during the cut flower senescence,the expression levels in various flower organs continually increased to a maximum at 6 d,and then declined sharply.At the same time,ethylene production increased slowly until 8 d,and then dramatically increased.Temporal and spatial expression of the OnERS1 gene suggested that its function may be related with flower senescence.

关 键 词：文心兰 乙烯受体 ETR1 ERS1 基因克隆 荧光定量PCR 

分 类 号：Q943.2[生物学—植物学] S682.31[农业科学—观赏园艺]