中间锦鸡儿CiWRKY2基因克隆与表达分析  

Cloning and Expression Analysis of CiWRKY2 from Caragana intermedia

作　　者：毛铭铢 万永青[1] 李国婧[1] 杨杞[1] 王瑞刚[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《分子植物育种》2017年第4期1280-1288,共9页Molecular Plant Breeding

基　　金：国家自然科学基金(31360169);内蒙古产业创新创业人才团队;内蒙古自治区科技创新团队(201503004)共同资助

摘　　要：通过RACE技术从中间锦鸡儿中克隆得到一个WRKY转录因子编码基因CiWRKY2,该基因g DNA为3 224 bp,含有4个内含子;cDNA长2 032 bp,开放阅读框1 725 bp,可以编码574个氨基酸,蛋白分子量大小约为63.29 kD,等电点为7.65。系统进化分析发现,CiWRKY2与豆科植物WRKY相似度最高,属于第一类WRKY蛋白。亚细胞定位显示CiWRKY2蛋白定位于细胞核。实时荧光定量结果表明CiWRKY2主要在叶、根中表达,并且该基因转录水平的表达受到干旱、盐碱、高温、低温和ABA诱导,表明CiWRKY2基因可能在植物抗逆中起到一定作用。A WRKY encoding gene was cloned by RACE technique from Caragana intermedia,designated as CiWRKY2.The g DNA of CiWRKY2 was 3 224 bp,containing four introns;the cDNA was 2 032 bp including a1 726 bp open reading frame,which encoded a 574-amino acid protein with an estimated molecular mass of 63.29 kD and an isoelectric point of 7.65.Phylogenetic analysis showed that CiWRKY2 shared high identity with the WRKY proteins from leguminous plants and belonged to groupⅠof the WRKY transcription factor family.CiWRKY2 localized in the nuclei and mainly expressed in leaves and roots.The expression patter analyzed by qRT-PCR showed that CiWRKY2 transcript was induced under drought,NaCl,high pH,cold,heat and ABA treatments.It suggested that CiWRKY2 might play a role in plant stress tolerance.

关 键 词：CiWRKY2 中间锦鸡儿 基因克隆 实时定量RT-PCR 

分 类 号：Q943.2[生物学—植物学]