过表达海马齿SpSOS1基因能增强转基因拟南芥的耐盐性  

Over-expression of a Sesuvium portulacastrum Salt Overly Sensitive Gene(SpSOS1) Enhances Salt Tolerance of Transgenic Arabidopsis

作　　者：范亚飞[1] 周扬[1] 万舒敏[1] 莫春演 江行玉[1] 

机构地区：[1]海南大学农学院,海南省热带生物资源可持续利用重点实验室,海口570228

出　　处：《分子植物育种》2017年第4期1347-1353,共7页Molecular Plant Breeding

基　　金：海南省科技重大专项基金(HNGDhs201502)资助

摘　　要：细胞膜Na^+/H^+逆转运蛋白SOS1在植物耐盐过程中起重要作用。本研究将盐生植物海马齿中的细胞膜Na^+/H^+逆转运蛋白基因SpSOS1构建到植物双元表达载体pCAMBIA1304上,采用农杆菌介导的方法将SpSOS1基因转入到拟南芥中,在含有50μg/mL潮霉素B的MS培养基上筛选转基因后代,结合目标基因和标记基因的PCR验证,获得了超表达的转基因拟南芥。利用T_3代转基因幼苗进行耐盐性分析。研究表明,转SpSOS1基因的拟南芥在50 mmol/L NaCl的培养基中发芽率在80%以上,明显高于野生型拟南芥的45%。转SpSOS1基因拟南芥幼苗的耐盐性明显提高,在100 mmol/L NaCl条件下能正常生长,具有较高的生物量、根长和侧根数,而野生型拟南芥在50 mmol/L NaCl的胁迫下明显萎蔫、失绿、甚至死亡。说明过表达SpSOS1基因显著提高了拟南芥的耐盐性。Plasma membrane Na^＋/H^＋ antiporter SOS1（salt overly sensitive） plays significant role in plant salt tolerance.In this study,a Na^＋/H^＋ antiporter SOS1 gene from Sesuvium portulacastrum was constructed into the p CAMBIA1304 binary vector,and the SpSOS1 gene was transformed into Arabidopsis by using the agrobacterium-mediated transformation.The SpSOS1 over-expressing transgenic plants were obtained by screened transgenic plants on MS medium containing 50 μg/m L Hygromycin B and confirmed by the PCR verification of target gene and marker gene.T3 transgenic seedlings were used to analyze the salt tolerance.The results showed that Arabidopsis over-expressing SpSOS1 germination rate was up to 80% under 50 mmol/L NaCl treatment,which obviously overtopped than wild type plants with the 45 percent.The salt tolerance of SpSOS1 transgenic plants significantly improved,and grew up normally under 100 mmol/L NaCl treatment with the high biomass,root length and lateral root number.However the wild type plants were wilted,degraded green even died.This study showed that over-expressing the Sesuvium portulacastrum SpSOS1 gene could enhance salt tolerance of transgenic Arabidopsis.

关 键 词：SpSOS1基因 转基因拟南芥 海马齿 耐盐性 

分 类 号：Q943.2[生物学—植物学]