水稻雌性半不育突变体M21的细胞学研究和基因的初步定位  

Cytological Studies and Preliminary Mapping of M21,a Female Semi-sterility Mutant in Rice

作　　者：龙思芳[1] 张大双 彭强 姜雪 雷月 宫彦龙 徐海峰 李佳丽 邓如玉 朱速松 

机构地区：[1]贵州师范大学,贵阳550001 [2]贵州省水稻研究所,贵阳550006

出　　处：《分子植物育种》2017年第4期1371-1377,共7页Molecular Plant Breeding

基　　金：贵州省农业科技体制改革与创新服务园区项目([201619);贵州省农业科学院研究生科研创新基金项目([2010]0009);贵州水稻现代农业产业体系技术体系项目(GZCYTX2016-06);"十二五"农村领域国家科技计划项目(2014AA10A-604-11);贵州省科技合作计划项目([2015]7074);贵州师范大学研究生创新基金([2015]23)共同资助

摘　　要：水稻雌配子的发育直接影响水稻的育性。由于雌配子败育的机理复杂,使得雌性不育的分子机理研究一直未取得突破性进展。本研究以EMS诱变获得的半不育突变体M21为研究材料,采用细胞学技术发现M21是由胚囊部分败育造成的。以M21//M21/N22回交群体BC1F1为作图群体全基因组分析,构建全长为2674.2 cM的遗传图谱,包含了189对SSR标记,平均遗传距离为14.1 cM;复合区间作图法(CIM)进行QTL定位,在第11号染色体检测到一个新的雌配子半不育QTL qPS-a,初定位在标记RM26761和RM26841之间,其贡献率为20.2%。本研究有助于进一步探究水稻雌性半不育的遗传机制。The development of female directly affects the fertility office （Oryza sativa）. The molecular and cellular mechanisms regulating female fertility in rice remain poorly understood as for it＇s a complex network. In this study, we identified a new stable genetic female semi-sterility mutant M21. Cytological studies showed the reduced spikelet fertility primarily caused by reduced embryo sac viability. A genetic linkage map covering 2 674.2 cM was constructed based on genome-wide analysis using a baekcross population of M21//M21/N22, the genetic map contained 189 SSR makers with an average interval of 14.1 cM; a loci on chromosome 11 between makers RM26761 and RM26841 conferring the female semi-sterility was detected, named qPS-a, which explained more than 20% of the phenotypic variation for female semi-sterility. No sterility related QTLs/genes were reported between these two markers. These results would be beneficial to the further study on the genetic mechanisms of female semi-sterility in Rice.

关 键 词：水稻 雌配子 半不育 基因定位 

分 类 号：S511[农业科学—作物学]