关黄柏醇提部位化学拆分组分分析及其对热证大鼠的影响  

Resolvability of Chemical Components in Phellodendri Amurensis Cortex and Their Effects on Heat Syndrome Rats

作　　者：张宏伟[1] 王嘉琪[1] 张婕[1] 薛娟[1] 匡海学[1] 王秋红[1,2] ZHANG Hong-wei WANG Jia-qi ZHANG Jie XUE Juan KUANG Hai-xue WANG Qiu-hong(Heilongjiang Provincial Key Laboratory of Effective Substance Basis of Traditional Chinese Medicine and Natural Medicine, Key Laboratory of Basis and Application Research of North Medicine, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China Guangdong Pharmaceutical University, Guangzhou 510224, China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室,哈尔滨150040 [2]广东药科大学,广州510224

出　　处：《中国实验方剂学杂志》2017年第10期29-33,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81473351);黑龙江中医药大学研究生创新科研项目(2015010)

摘　　要：目的:探讨关黄柏醇提部位化学成分拆分的合理性及各拆分组分对热证大鼠的作用。方法:选用95%乙醇提取,结合乙酸乙酯萃取、大孔吸附树脂和阳离子交换树脂等处理对关黄柏醇提部位化学组分进行拆分,拆分组分采用UPLC-QTOF/MS分析并进行主成分分析;采用左甲状腺素钠片诱导大鼠热证模型,观察各拆分组分对热证大鼠中枢神经递质及能量代谢相关物质含量的影响。结果:关黄柏经过95%乙醇提取、乙酸乙酯萃取、大孔树脂和阳离子交换树脂处理得到生物碱组分、非生物碱组分和乙酸乙酯组分,产率分别为0.65%,4.70%,2.50%;主成分分析各拆分组分分布集中在非常明显的不同区域上,各组分在成分组成上存在非常明显的差别,各拆分组分间主要药效成分基本无交叉;关黄柏醇提部位各拆分组分可在不同程度上降低热证大鼠血清及肝脏中与中枢神经递质及能量代谢相关的递质、激素、酶类的含量,改善热证大鼠中枢神经递质释放和能量代谢。结论:关黄柏醇提部位发挥"寒"药性的药效物质基础主要为生物碱组分和乙酸乙酯组分。Objective： To investigate the resolvability of chemical constituents in Phellodendri Amurensis Cortex and their effects on heat syndrome rats. Method： We split the chemical constituents in Phellodendri Amurensis Cortex with 95% ethanol extraction,ethyl acetate extraction,macroporous resin and cation exchange resin separation. We established the total ion current fingerprint of these chemical constituents by UPLC-Q-TOF/MS for principal component analysis（PCA）. We observed effect of the spilt components in Phellodendri Amurensis Cortex on central nervous system neurotransmitter and energy metabolism of heat syndrome rats induced by levothyroxine sodium tablets. Reslut： The chemical constituents in Phellodendri Amurensis Cortex were split three components, namely, alkaloid component, non-alkaloid component and ethyl acetate component, their yields were 0. 65%,4. 70% and 2. 50%. The chemical components in Phellodendri Amurensis Cortex were proved basically no cross by PCA. The chemical components in Phellodendri Amurensis Cortex should decrease the contents of neurotransmitters,hormones and enzymes related to central neurotransmitters and energy metabolism in the serum and liver of heat syndrome rats with different degrees. Conclusion： The material basis of ‘cold＇nature of Phellodendri Amurensis Cortex are mianly for alkaloid component and ethyl acetate component.

关 键 词：关黄柏 化学拆分组分 超高效液相色谱串联飞行时间质谱 热证 左甲状腺素钠片 生物碱 

分 类 号：R283.6[医药卫生—中药学] R284[医药卫生—中医学]