新疆银叶真藓居群nrDNA ITS序列的遗传分化  

Genetic Differentiation of nr DNA ITS Sequence of Bryum argenteum Hedw.(Bryaceae) Populations in Xinjiang Uygur Autonomous Region

作　　者：汪琛颖[1] 买买提明.苏来曼 

机构地区：[1]郑州师范学院生命科学学院,郑州450044 [2]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《分子植物育种》2017年第4期1426-1435,共10页Molecular Plant Breeding

基　　金：河南省教育厅高等学校重点科研项目(16A180053);河南省科技计划资助项目(132300410358);国家自然科学基金项目(31460048;31660052)共同资助

摘　　要：银叶真藓是世界广布种。对于分布在中国新疆维吾尔自治区不同地理居群的银叶真藓的遗传结构及遗传多样性的研究还未见报道。本研究以采集的新疆地区18个居群的银叶真藓共47株植物样品,采用改良的快速提取法提取随机单株植物总DNA,运用分子生物学技术分析银叶真藓居群的遗传多样性。研究结果表明:新疆不同地理居群银叶真藓nrDNA ITS序列可分为35种单倍型,存在537个变异位点;分子变异分析(AMOVA)显示有88.76%的遗传变异发生在居群间水平,居群内部的遗传变异为11.24%,银叶真藓居群间的遗传分化程度大于居群内的遗传分化。居群遗传变异的分化系数(Fst)为0.887 59,基因流值(Nm)为0.063 3,由于Nm值小于1,可以说明各居群间的基因流低。单倍型多态性水平(Hd)为0.982±0.010,核苷酸多态性水平(Pi)为0.170 87±0.028 67。Bryum argenteum Hedw.is a cosmopolitan moss.We have no information on its genetic diversity and population genetic structure in Xinjiang Uygur Autonomous Region,China.Therefore,the present population genetic study was performed to provide data on population genetic structure,potential gene flow in B.argenteum in Xinjiang.We studied 47 randomly selected plants from 18 geographical populations by using the nr DNA internal transcribed spacer（ITS） molecular markers.The results showed that in the 18 B.argenteum populations there were total of 35 haplotypes,and 537 variable sites existed.ITS sequence analysis of molecular variance（AMOVA）showed that genetic variation among populations was 88.76%,genetic variation within populations was 11.24%,and the degree of genetic differentiation among populations was greater than the genetic differentiation within populations.Genetic differentiation（Fst） was 0.887 59;Nm=0.063 3,Nm〈1,indicated B.argenteum of Xinjiang had limited gene flow among these populations.The haplotype diversity level was from 0.972 to 0.992.The nucleotide diversity level ranged from 0.142 2 to 0.199 54.

关 键 词：银叶真藓 NRDNA ITS 遗传分化 

分 类 号：Q943.2[生物学—植物学]