猫儿屎的愈伤组织诱导和植株再生  

Callus Induction and Plant Regeneration of Decaisnea insignis

作　　者：魏文桃 陈芸芸[1] 叶晗[1] 杨林森 梁宏伟[1] 

机构地区：[1]三峡大学生物技术研究中心,宜昌443002 [2]神农架金丝猴保育生物学湖北省重点实验室,神农架442421

出　　处：《分子植物育种》2017年第4期1473-1477,共5页Molecular Plant Breeding

基　　金：国家"十二五"科技支撑计划课题项目(2013BAD03B03)资助

摘　　要：以猫儿屎的幼嫩种子作为外植体,研究不同植物生长调节剂对猫儿屎不定芽诱导和增殖影响,建立猫儿屎不定芽增殖及植株再生的高频再生体系。结果表明:幼嫩种子在MS+0.1 mg/L KT+2.0 mg/L 2,4-D培养基中愈伤组织诱导率最高,可达92%;不定芽的分化增殖的最适培养基为1/2 MS+0.5 mg/L NAA+3.0 mg/L6-BA,繁殖系数可达14.49~19.32;不定芽生根诱导的最适培养基为1/2 MS+0.6 mg/L NAA,生根率可达79.83%,根系和小植株长势良好;完整小植株经炼苗后移栽到混合基质(腐植质:细河沙=1:1)中,成活率可达90%。In order to investigate the effects of various plant growth regulator on the induction of Adventitious bud and proliferation of Decalsnea insignis, immature seed were used as explants for tissue cultural experiment, and massive propagation and regeneration plants were obtained to establish a high-frequency regeneration system of Decaisnea insignis. The results showed that the optimum medium for callus induction of immature seeds was MS medium supplemented with 0.1mg/L KT and 2.0 mg/L 2,4-D, and the induction rate reached to 92%. The optimum medium of adventitious shoot propagation was 1/2 MS medium supplemented with 0.5 mg/L NAA and 3.0 mg/L 6-BA, the reproduction coefficient reached to 14.49-19.32. The optimum Medium for Rooting was 1/2 MS medium supplemented with 0.6 mg/L NAA, and the rooting rate was 79.83%, the root system and small plants grew well. The complete regenerated plants were transplanted to the mixed matrix （humus： river sand = 1：1） after domestication, and the survival rate could reached 90%.

关 键 词：猫儿屎 愈伤组织 不定芽 植株再生 

分 类 号：S567.19[农业科学—中草药栽培]