大黄厚朴组分配伍对大鼠AP的治疗作用及其机制的影响  

Effect of Combined Administration of Rhei Radixet Rhizoma and Magnoliae Officinalis Cortexin Treating Rat AP and Its Mechanism

作　　者：陈银芳[1,2] 余日跃[1,3] 业康 聂斌[1] 彭红[1] 徐国良 CHEN Yin-fang YU Ri-yue YE Kang NIE Bin PENG Hong XU Guo-liang(Jiangxi University of Traditional Chinese Medicine （ TCM）, Nanchang 330004, China Hunan University of Chinese Medicine, Changsha 410208, China Key Laboratory of Pharmacology of TCM of Jiangxi, Nanchang 330004, China)

机构地区：[1]江西中医药大学,南昌330004 [2]湖南中医药大学,长沙410208 [3]江西省中药药理学重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2017年第10期122-126,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160424);国家重点基础研究发展计划(973计划)项目(2010CB530603);江西省中医药科技计划项目(2015A247)

摘　　要：目的:研究大黄厚朴中大黄素、芦荟大黄素、厚朴酚、和厚朴酚4种主要成分配伍组方对急性胰腺炎(acute pancueatitis,AP)大鼠的治疗作用及其可能的机制。方法:采用均匀设计法将4种组分配成C^H 6个配伍方。采用牛磺胆酸钠逆行注射胰胆管法,建立急性胰腺炎大鼠模型。大鼠术后15 min给药,24 h腹主动脉取血,测定血清中淀粉酶,胰脂肪酶,白细胞介素-6(IL-6),IL-10,肿瘤坏死因子-α(TNF-α)含量的变化。另取胰腺、肺组织行病理切片检查,蛋白免疫印迹(Western blot)法检测肺组织中核因子-κB(NF-κB)蛋白的表达。结果:各治疗组大鼠血清淀粉酶、胰脂肪酶,IL-6,TNF-α含量与模型组比较,均有显著下调(P<0.05,P<0.01);IL-10含量亦有所下降,但仅H组统计有显著降低(P<0.01)。病理检查结果显示,模型组大鼠胰腺组织大量片状坏死,胰腺腺泡结构不清;肺泡腔明显变小,肺泡上皮出现增生,间隔显著增大;而各治疗组胰腺和肺组织病灶坏死明显有所减轻。各治疗组肺组织中NF-κB蛋白表达与模型组比均显著下调。结论:大黄厚朴4种药效成分大黄素、芦荟大黄素、厚朴酚、和厚朴酚配伍组方对大鼠急性胰腺炎有明显治疗作用,其机制可能与NF-κB蛋白调节,炎症因子介导有关。Objective： To study the effect of combined administration of Rhei Radixet Rhizoma and Magnoliae Officinalis Cortex in treating acute pancreatitis（AP） rats and its possible mechanism. Method： The SD rats were randomly divided into four groups,including sham-operated group,model group and six treatment groups（C-H）. The rat model of acute pancreatitis was established by the retrograde injection of sodium taurocholate. At 15 min after injection,the rats were given drugs. Abdominalaortic blood was collected at 24 hours for determing serum amylase,pancreatic lipase, interleukelin-6（IL-6）,IL-10 and tumor necrosis fuctor-α（TNF-α） content. Pancreasand lung tissues were sent tothepathological examination. Western blot method was used for detecting nucleax factor-κB（NF-κB） protein expression in lung tissues. Result： Compared with model group,amylase,pancreatic lipase,IL-6,TNF-α,NF-κB protein expression in lung tissues in treatment groups were significantly decreased（P〈0.01,P〈0. 05）. But the IL-10 was significantly decreased（P〈0.01） only in group H. The pancreas in model group rats showed large necrosis and unclear structures of pancreatic acinar,and notably smaller alveolar cavity,alveolar epithelial hyperplasia and significantly increased interval. This situation had been greatly alleviated in treatment groups. Besides,NF-κB protein expression in lung tissues of treatment groups was remarkably lower than model group. Conclusion： Components in Rhei Radixet Rhizoma and Magnoliae Officinalis Cortex have an effect in treating acute pancreatitis,and its mechanism may be related to NF-κB protein regulation and inflammatory cytokines mediation.

关 键 词：急性胰腺炎 大黄素 芦荟大黄素 厚朴酚 和厚朴酚 

分 类 号：R285.5[医药卫生—中药学]