赤霉素对白刺花幼苗生长发育的影响  

Influence of Gibberellin on the Growth and Development of Sophora davidii Seedlings

作　　者：刘杰[1,2] 吴雪花[1] 罗充[1] 孙威[1,2] 汤晓辛[1,2] 乙引[1,2] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550001 [2]贵州省植物生理与发育调控重点实验室,贵阳550001

出　　处：《分子植物育种》2017年第4期1515-1521,共7页Molecular Plant Breeding

基　　金：贵州省植物生理与发育调控重点实验室建设;贵州省重点实验室建设项目(黔科合计Z字[2011]4005);教育部喀斯特山地生物多样性保护与可持续利用创新团队;长江学者和创新团队发展计划项目(IRT1227);贵州师范大学博士科研启动项目(11904-0514012);贵州省科技厅-贵州师范大学联合基金(黔科合LH字[2016]7210号)共同资助

摘　　要：实验采用不同浓度(0,0.05 mg/L,0.1 mg/L,0.2 mg/L,0.4 mg/L,0.6 mg/L,0.8 mg/L,1.0 mg/L)的赤霉素(GA)对白刺花(Sophra davidii)幼苗进行处理,以探讨GA对白刺花(Sophra davidii)幼苗生长发育的影响。结果表明:不同浓度的GA均可促进白刺花根长的伸长,以0.6 mg/L GA处理12 d主根最长;随着GA处理浓度的增加,茎长的呈现先升高后降低的趋势,0.05 mg/L的GA处理12 d茎长最长;0.05 mg/L的GA处理12 d侧根数目的增多更为显著;随着GA浓度的升高,真叶数目也不断增加,以0.6 mg/L GA处理12 d真叶数目最多。研究还发现:随着GA浓度的升高,短期处理(3 d)叶绿素a、b含量以及POD活性在0.2 mg/L GA时最高,电导率在0.4 mg/L GA时最高;中期处理(6 d)CAT、POD活性在0.6 mg/L GA时最高;长期处理12 d;叶绿素a、b含量在0.4 mg/L时最高,电导率在0.6 mg/L GA时最高。In this research,different concentrations（0,0.05 mg/L,0.1 mg/L,0.2 mg/L,0.4 mg/L,0.6 mg/L,0.8 mg/L and 1.0 mg/L） of gibberellin（GA） were used to treat the seedlings of Sophora davidii so as to explore the effect of GA on the growth and development.Results showed that GA,at different concentrations,could accelerate the elongation of the roots of Sophora davidii.The main root of the Sophora davidii seedlings treated by GA at a concentration of 0.6 mg/L for 12 d was found to be the longest.With increasing concentration of GA,the stem length tended to firstly increase and then reduce,and was shown to be at its maximum after 12 d of the treatment using 0.05 mg/L of GA.In addition,the number of lateral roots increased the most in this treatment group.As the concentration of GA increased,the number of euphyllae increased,and was at its maximum after being treated for 12 d using 0.6 mg/L of GA.Besides,the study also revealed that,with the growing concentration of GA,the chlorophyll a and b contents and the peroxidase activity（POD） were the highest in a short-term treatment（3 d） using0.2 mg/L of GA,while the highest conductivity was found in the group treated with 0.4 mg/L of GA.For mediumterm treatment（6 d）,the highest activities of catalase（CAT） and POD were found in seedlings treated with 0.6 mg/L of GA.The highest chlorophyll a and b contents,and the highest conductivity,were observed in groups treated with 0.4 mg/L and 0.6 mg/L of GA for 12 d,separately.

关 键 词：赤霉素 白刺花 外部形态 生理指标 

分 类 号：Q945[生物学—植物学]