橡胶树三个品种的核型分析  

Karyotype Analysis of Three Varieties in Hevea brasiliensis

作　　者：马梦晨[1] 王英[1,2] 高和琼[1] 庄南生[1,2] 

机构地区：[1]海南大学农学院,海口570228 [2]海南省热带生物资源可持续利用重点实验室,海口570228

出　　处：《分子植物育种》2017年第4期1563-1568,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31260185);中央财政支持中西部高校提升综合实力专项资金(M8K312400100500-3003)共同资助

摘　　要：文昌193、红星1、海垦2是广泛种植的优良橡胶品种,对其核型进行研究将在橡胶树育种中具有重要意义。以古铜期嫩叶为试验材料,采用酶解去壁低渗法对文昌193、红星1、海垦2的染色体核型进行研究。结果表明,文昌193的染色体核型公式2n=36=30m+6sm,相对长度组成为2n=36=8S+8M1+16M2+4L,核不对称系数为61.22%,红星1的染色体核型公式2n=36=32m(2sat)+4sm,相对长度组成为2n=36=6S+12M1+14M2+4L,核不对称系数为59.76%,海垦2的染色体核型公式2n=36=32m(2sat)+4sm,相对长度组成为2n=36=6S+10M1+16M2+4L,核不对称系数为58.61%,三者核型类型均为2B型,均属于对称程度较高的染色体组。该研究可为橡胶树育种、种质资源鉴定和基因定位等研究提供细胞学基础。Wenchang 193,Hongxing 1 and Haiken 2 were large-scale of the extension cultivation of choiceness,and their karyotype analysis was important in breeding.Karyotypes of Wenchang 193,Hongxing 1 and Haiken 2were analysed with the enzyme and low osmotic method by using the tender leaves as material.The results indicated that the karyotype formula of Wenchang 193 was 2n=36=30m＋6sm,the chromosome composition of relative length was 2n=36=8S＋8M1＋16M2＋4L,the asymmetrical karyotype coefficient was 61.22%,and the karyotype formula of Hongxing 1 was 2n=36=32m（2sat）＋4sm,the chromosome composition of relative length was 2n =36 =6S＋12M1＋14M2＋4L,the asymmetrical karyotype coefficient was 59.76%,as to Haiken 2,the karyotype formula was 2n=36=32m（2sat）＋4sm,the chromosome composition of relative length was 2n=36=6S＋10M1＋16M2＋4L,the asymmetrical karyotype coefficient was 58.61%,the karyotypes of three varieties were all belong to 2B type.The study would provide cytological basis for the breeding,germplasm analysis and gene location of Hevea brasiliensis.

关 键 词：橡胶树 文昌193 红星1 海垦2 核型分析 

分 类 号：S794.1[农业科学—林木遗传育种]