三七粉调血脂作用及机制研究  

Hypolipidemic effect of powder of Panax notoginseng and its mechanism

作　　者：董婧婧[1,2] 刘艳菊[1,2] 涂济源 付伟[1,2] 邓婕[3] 瞿领航 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065 [2]湖北省中药炮制工程技术研究中心,湖北武汉430065 [3]湖北中医药大学黄家湖医院,湖北武汉430065

出　　处：《中草药》2017年第8期1597-1603,共7页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局2015年中医药部门公共卫生专项资金(中药科技类)项目"中药炮制技术传承基地"(湖北中医药大学基地)

摘　　要：目的研究三七粉对高脂血症模型大鼠的调血脂作用及其作用机制。方法采用高脂饲料配合高脂乳剂诱导高脂血症大鼠模型,高脂血症大鼠分别ig给予高、中、低剂量(1.08、0.54、0.27 g/kg)三七粉,以脂必妥(37.8 mg/kg)和辛伐他汀(1.8 mg/kg)为阳性对照,各组每天给药1次,连续给药8周。给药结束后测定各组大鼠血清中总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白-胆固醇(LDL-C)水平,同时检测血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)活性;对肝组织进行石蜡切片和HE染色,观察组织病理变化;采用RT-PCR法检测肝组织中低密度脂蛋白受体(LDLR)、组蛋白/非组蛋白去乙酰化酶(沉默调节因子1,SIRT1)和肝X受体α亚型(LXR-α)的基因表达,同时采用Western blotting法检测肝组织中胆固醇调节元件结合蛋白-2(SREBP-2)及SREBP裂解激活蛋白(SCAP)的表达水平。结果三七粉显著降低高脂血症大鼠血清中TC、TG和LDL-C水平及AST、ALT活性;组织学观察结果显示三七粉明显减轻了肝损伤和脂肪肝;分子水平结果显示,三七粉能上调LDLR和SIRT1、下调LXR-α基因表达。同时,三七粉显著降低了SREBP-2和SCAP的蛋白表达。结论三七粉具有调血脂、保护肝脏的作用,这可能与三七粉上调SIRT1、下调LXR-α基因表达,进而下调SCAP/SREBP-2信号通路抑制胆固醇合成,以及上调LDLR的基因表达提高肝脏对血液循环中LDL-C的摄取的机制有关。Objective To investigate the hypolipidemic effects of powder of Panax notoginseng（PPN） and explore its possible mechanism. Methods Hyperlipidemic rats model was established, and orally given three dosages of PPN for 8 weeks. The levels of serum ALT, AST, TC, TG, and LDL-C were detected. The pathological changes of liver tissues were observed by HE staining. Gene expressions of hepatic low density lipoprotein receptor（LDLR）, SIRT1, and LXR-α were measured with RT-PCR analysis. Protein expression of SREBP-2 and SCAP was determined by Western blotting. Results Three dosages of PPN significantly decreased serum ALT, AST, TC, TG, and LDL-C levels. Histological data indicated that PPN notably reduced liver injury and hepatic steatosis in hyperlipidemic rats. In molecular study, m RNA expression of hepatic LDLR and SIRT1 was up-regulated and LXR-α gene expression was down-regulated in PPN treated rats. Additionally, PPN significantly reduced protein expression of SREBP-2 and SCAP. Conclusion The positive effect of PPN on hyperlipidemic rats may be related to the inhibition of cholesterol synthesis of PPN through the up-regulation of SIRT1 and down-regulation of LXR-α and SCAP/SREBP-2 signaling pathway. Additionally, PPN could up-regulate hepatic LDLR m RNA expression and improve uptake of LDL-C in circulation.

关 键 词：三七粉 调血脂 低密度脂蛋白受体 SIRT1 LXR-α SCAP SREBP-2 

分 类 号：R285.5[医药卫生—中药学]