野生与栽培夏枯草5种活性成分的HPLC测定  

Determination of five bioactive constituents in wild grown and cultivated Prunella vulgaris by HPLC

作　　者：皮胜玲 张凯强[1,2,3] 胡玉珍[1,2,3] 黄杰[1,2,3] 李亚梅[1,2,3] 夏伯候[1,2,3] 林丽美[1,2,3] 吴萍[1,2,3] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410203 [2]湘产大宗药材品质评价湖南省重点实验室,湖南长沙410203 [3]湖湘中药资源保护与利用协同创新中心,湖南长沙410203

出　　处：《中草药》2017年第8期1666-1670,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81503041);国家科技部公益性行业专项(201507002);湖南省自然科学基金资助项目(13JJ4089);湖湘青年科技创新创业平台资助项目(2013);2016CX09湖南中医药大学研究生创新课题

摘　　要：目的建立同时测定夏枯草Prunella vulgaris中迷迭香酸、异迷迭香酸苷、咖啡酸、芦丁、木犀草素5种活性成分的方法,并对不同产地野生与栽培夏枯草进行测定研究,比较夏枯草野生品与栽培品在酚酸和黄酮类成分量上的差异。方法采用Agilent 5HC-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸水溶液(B)为流动相;柱温30℃;体积流量1.0 mL/min;检测波长280 nm。以独立样本t检验,辅以聚类分析和相关性分析方法进行评价。结果独立样本t检验表明,夏枯草野生品与栽培品在迷迭香酸、芦丁、咖啡酸和木犀草素4种成分量上差异显著(P<0.01),而异迷迭香酸苷量无差异(P>0.05)。聚类分析结果显示,大部分产地野生与栽培夏枯草能被正确区分。相关性分析结果表明,果穗长度与夏枯草主要成分量无明显关系。结论所建立的测定方法简单、可行,可以作为夏枯草品质评价方法之一。Objective To compare the contents of phenolic acids and flavonoids in wild grown and cultivated Prunella vulgaris from various habitats, an HPLC method was established for simultaneous determination of five bioactive compounds（rosmarinic acid, salviaflaside, caffeic acid, rutin, and luteolin）. Methods The Agilent 5HC-C18（250 mm × 4.6 mm, 5 μm） was adopted with a gradient eluent system composed of acetonitrile and 0.1% phosphoric acid aqueous solution at the temperature of 30 ℃. The flow rate was 1.0 m L/min. The detection wavelength was 280 nm. Independent t-test（t-test）, hierarchical clustering analysis（HCA）, and correlation analysis were applied to analyzing and evaluating wild grown and cultivated P. vulgaris. Results The t-test results showed that the contents difference of rosmarinic acid, rutin, caffeic acid, and luteolin had statistical significance（P〈0.01）. However, there was no obvious difference for salviaflaside（P〉0.05）. According to the result of HCA, most of the cultivated and wild materials could be differentiated. The result of correlation analysis showed that the ear length has no significant influence on the contents of main compounds in P. vulgaris. ConclusionThe determination method is simple and feasible, and it can be used as one of the quality evaluation method of P. vulgaris.

关 键 词：夏枯草 HPLC 聚类分析 相关性分析 迷迭香酸 异迷迭香酸苷 咖啡酸 芦丁 木犀草素 

分 类 号：R286.6[医药卫生—中药学]