慢病毒介导的Math1基因感染神经干细胞研究  

The study of neural stem cells infected by recombinant lentivirus with Math1

作　　者：潘松 陈绮琴 钱学贞 张松 周军 Pan Song Chen Qiqin Qian Xuezhen et al(Guangming New District People’ s Hospital in Shenzhen （Shenzhen 518106)

机构地区：[1]广东省深圳市光明新区人民医院,深圳518106

出　　处：《陕西医学杂志》2017年第5期554-555,共2页Shaanxi Medical Journal

基　　金：广东省深圳市医疗卫生类科技计划项目(201303252)

摘　　要：目的:探讨慢病毒携带目的基因Math1感染神经干细胞的适宜条件及其影响。方法:以不同感染复数(MOI)的慢病毒分别感染神经干细胞,确定最适感染条件。无血清培养液继续培养,观察感染效率有无下降。结果:随着MOI的增加,慢病毒的效率效率逐渐增加;当MOI为10时,感染效率达(83.83±1.49)%。无血清培养液继续培养30d后,感染效率达(81.27±1.48)%,与继续培养前比较无明显下降(P>0.05)。结论:携带目的基因的重组慢病毒可成功感染神经干细胞,被感染的神经干细胞的生长无明显改变。Objective：To explore suitable condition of NSC infected by lentivirus with Math1,growth of NSC influenced by lentivirus.Methods：To determine suitable infection condition by using lentivirus with different MOI to infect NSC.To explicit whether infected ratio reduced when infected NSC were culture by serum-free medium.Results：With the increase of MOI,infected efficiency gradually increased.Infected efficiency reached（83.83±1.49）%when MOI was 10.After sequentially cultured 30 days,infected efficiency reached（81.27±1.48）% which had no significantly decline compared to 30 days ago.Conclusion：Recombinant lentivirus with target gene could successfully infect neural stem cells,and there has no significant change in the growth of infected NSC.

关 键 词：干细胞 慢病毒属 感染 @携带目的基因Math1 

分 类 号：R392.2[医药卫生—免疫学]