圆口铜鱼精浆的理化性质分析  

Analysis of physical and chemical properties of seminal plasma of Coreius guichenoti

作　　者：王昌霖[1,2] 吴兴兵[1] 朱永久[1] 杨德国[1] 王亚龙[1,3] 陈亮[1,3] 杨少荣[4] 王小明[4] WANG Chang-lin WU Xing-bing ZHU Yong-jiu YANG De-guo WANG Ya-long CHEN Liang YANG Shao-rong WANG Xiao-ming(Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223 , China College of Aquaculture, Huazhong Agricultural University, Wuhan 430070, China College o f Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China China Three Gorges Corporation, Chengdu 610041, China)

机构地区：[1]中国水产科学研究院长江水产研究所,农业部淡水生物多样性保护重点实验室,武汉430223 [2]华中农业大学水产学院,武汉430070 [3]上海海洋大学水产与生命学院,上海201306 [4]中国长江三峡集团公司,成都610041

出　　处：《淡水渔业》2017年第3期41-44,共4页Freshwater Fisheries

基　　金：农业部公益性行业科研专项经费项目(201203086-02);中国长江三峡集团公司科研专项(JGJ0252013)

摘　　要：对圆口铜鱼(Coreius guichenoti)精浆离子和氨基酸成分及精液生理特性进行了检测分析。结果显示,圆口铜鱼精液pH值为7.3,呈弱碱性;精液浓度为39.7%,精子密度为5.3×10~9个/mL;精浆离子以Na^+含量88.7 mmol/L最高,其次是K^+,之后为Ca^(2+)、Mg^(2+)、Zn^(2+)、Cu^(2+);精浆水解氨基酸总量为2 872.69μmol/100mL,其中以脯氨酸含量最高,蛋氨酸、酪氨酸和组氨酸含量最低。该结果填补了圆口铜鱼繁殖生物学的相关数据,为圆口铜鱼规模化人工繁育提供了基础资料。This paper focused on the main physiological characteristics of semen, seminal plasma ion and amino acid com-position of Coreius guichenoti. The results showed that the semen pH and semen concentration of C. guichenoti were at the range of 7. 3 and 39. 7% , respectively, and which was weakly alkaline. The sperm density was 5. 3 × 10^9 ind. / mL. The highest content in plasma was Na＋ with the concentration of 88. 7 mmol/ L, then followed by K ＋ , Ca2＋ , Mg2＋ , Zn2＋ , Cu2 ＋. The total amount of hydrolyzed amino acids in seminal plasma was 2 872. 69 μmol/ 100 mL, and the content of pro-line was the highest, and that of methionine, tyrosine and histidine were the lowest. The results could enrich the research contents of fish sperm biology and provide basic theory support for large - scale artificial breeding and species protection.

关 键 词：圆口铜鱼(Coreius guichenoti) 精液浓度 精子密度 精浆离子 水解氨基酸 

分 类 号：S917.4[农业科学—水产科学]