奇异变形杆菌中赖氨酸-2-羟基异丁酰化蛋白的分析  

Analysis of lysine 2-hydroxyisobutyrylation proteins in Proteus mirabilis

作　　者：董瀚阳 郭振昌[1] 田姗姗[1] 翟贵金[1] 张锴[1] 

机构地区：[1]天津市医学表观遗传重点实验室天津医科大学生物化学与分子生物学系天津市医学表观遗传协同创新中心,天津300070

出　　处：《色谱》2016年第12期1215-1218,共4页Chinese Journal of Chromatography

基　　金：国家重大科学研究计划(2012CB910601);国家自然科学基金项目(21275077);天津市应用基础与前沿技术研究计划项目(14JCYBJC24000)~~

摘　　要：蛋白质的赖氨酸修饰广泛参与基因调控、转录、代谢等重要的生物过程。在真核细胞组蛋白上发现了一种新的赖氨酸修饰——2-羟基异丁酰化,这种修饰对于生殖细胞分化具有调控功能。该研究旨在探索这种修饰在原核生物非组蛋白中的特征。通过亲和富集、高效液相色谱-串联质谱鉴定和生物信息学分析,在奇异变形杆菌中鉴定到大量未见报道的2-羟基异丁酰化蛋白及其位点,进而考察了原核生物中2-羟基异丁酰化修饰蛋白的分布特征、分子网络和通路特点。研究表明,赖氨酸-2-羟基异丁酰化在原核生物中具有广泛的分布,其生物学意义值得进一步研究。Protein lysine modifications play important roles in gene regulation, transcription, metabolism and other biological processes. Lysine 2-hydroxyisobutyrylation on histones has recently been discovered as a novel protein modification, and this modification was associated with germ cell differentiation. The problem whether the novel modification may or may not exist in prokaryotes was investigated. A number of 2-hydroxyisobutyrylated proteins and sites were identified in Proteus mirabilis using affinity enrichment, high performance liquid chromatography -tandem mass spectrometry (HPLC-MS/MS) and bioinformatics. The modified proteins were further characterized according to protein distribution properties, interaction networks and pathways. The results indicated that lysine 2-hydroxyisobutyrylation is widely distributed in prokaryotes, and may be significant in biological functions in prokaryotes.

关 键 词：高效液相色谱-串联质谱 翻译后修饰 2-羟基异丁酰化 赖氨酸 奇异变形杆菌 

分 类 号：O658[理学—分析化学]