一种新型核壳型色谱固定相在高效液相色谱中的评价及应用  

Evaluation and application of a new core-shell chromatographic stationary phase for high performance liquid chromatography

作　　者：孙元社 瞿其曙[3] 于淑新 李霜霜 唐涛 李彤 

机构地区：[1]大连依利特分析仪器有限公司,辽宁大连116023 [2]辽宁大连依利特分析仪器工程技术研究中心,辽宁大连116023 [3]安徽建筑大学材料与化学工程学院,安徽合肥230601

出　　处：《色谱》2016年第12期1249-1253,共5页Chinese Journal of Chromatography

基　　金：国家重大科学仪器设备开发专项(2012YQ12004403);国家自然科学基金(51472121);大连市科技计划项目(2015A11GX028)~~

摘　　要：采用扫描电镜、透射电镜和氮气吸附法对制备的新型核壳型色谱固定相进行了表征,结果表明该固定相单分散性好、表面放射状孔道壳层结构均一、孔径分布窄。对该核壳材料的表面进行C18键合修饰,考察其基本色谱性能,色谱柱的理论塔板数超过150 000块/m,色谱峰峰形对称,甲苯与乙苯的保留因子之比为1.45,亚甲基选择性优异。将该核壳材料应用于汽车尾气中醛酮类化合物的检测,在优化的色谱条件下,2,4-二硝基苯肼(DNPH)衍生的醛酮类化合物在15 min内获得了较好的分离效果。该核壳型C18色谱固定相具有分离速度快、选择性好、柱效高等特点,适于复杂样品的高效、快速分离分析。A new core-shell chromatographic stationary phase was characterized by scanning electron microscope, transmission electron microscope and nitrogen adsorption method. The results showed that it had excellent monodispersity,, homogeneous structure and narrow pore size distribution. It was modified by C18, and the chromatographic performance was also performed. The number of theoretical plates was over 150 000 plates/m, and the chromatographic peaks were symmetrical. The retention factor ratio of toluene and ethylbenzene was 1. 45, and the methylene selectivity was excellent. Finally, it was applied to detect aldehydes and ketones in automobile exhaust. The aldehydes and ketones which derivatized by 2, 4-dinitrophenyl-hydrazine ( DNPH) were well separated in less than 15 min under the optimized chromatographic conditions. The high separation speed, good selectivity and perfect column efficiency are obtained with the novel core-shell chromatographic stationary.

关 键 词：高效液相色谱 核壳型色谱固定相 评价与应用 醛酮类化合物 

分 类 号：O658[理学—分析化学]