没食子鞣酸对日本血吸虫感染小鼠模型肝病理改变的影响及作用机制研究  

Anti-fibrotic effect and mechanism of Gallic tannin on the hepatic fibrosis mouse model infected with Schistosoma japonicum

作　　者：李尤玲[1] 杨靖[2,3] 李健[2,3] 李刚[2] 

机构地区：[1]湖北医药学院附属人民医院中西结合科,湖北十堰442000 [2]湖北医药学院附属人民医院感染性疾病科,湖北十堰442000 [3]湖北医药学院基础医学院病原学实验室,湖北十堰442000

出　　处：《安徽医药》2017年第4期615-618,共4页Anhui Medical and Pharmaceutical Journal

基　　金：湖北省卫生计生血防专项(WJ2015XB032);十堰市科技局市级科学技术研究与开发专项基金项目(16K70);湖北医药学院研究生启动金资助计划项目(2015QDJZR08)

摘　　要：目的在建立血吸虫肝纤维化小鼠模型基础上,研究没食子鞣酸对血吸虫感染所致肝纤维化的影响及作用机制。方法利用日本血吸虫尾蚴感染BALB/c小鼠建立感染模型,将50只小鼠随机分为没食子鞣酸高、中、低浓度组、正常对照组和模型组等5组,每组10只。血吸虫感染4周后,各组分别给予相应的药物干预,模型组、正常对照组仅给予生理盐水,不予药物干预。没食子鞣酸连续灌胃4周后,观察小鼠的生活状况并记录小鼠体质量。实验开始8周后处死小鼠取标本做免疫组化检测结缔组织生长因子(CTGF)的表达;HE染色、Masson染色观察小鼠肝脏病理学变化。结果与造模组小鼠相比,3个没食子鞣酸干预组肝肉芽肿面积较小,纤维化程度较轻,肝组织CTGF蛋白表达水平较低,均差异有统计学意义(P<0.05)。结论没食子鞣酸可明显减轻日本血吸虫对小鼠感染模型肝病理学损伤,缓解肝肉芽肿所致肝纤维化程度。Objective To investigate the anti-fibrotic effect of Gallic tannin in a schistosomiasis-induced hepatic fibrosis mouse model. Methods Fifty BALB/c mice were randomly assigned into Gallic tannin high concentration group, middle concentration group, low concentration group,model group and normal group. The first four groups were infected by Schistosoma japonicum cercaria. After 4 weeks infection, the groups were given appropriate medication, control group received saline. Meanwhile, each group had intragastric ad-ministration one time every day for 4 weeks. Then we observed living conditions, and recorded the mice body weight. Specimens were de-tected by immunohistochemistry the expression of CTGF,HE staining,MASSON staining pathological changes in the liver of mice. Re-sults The degree of fibrosis,the expression of CTGF protein in the three Gallic tannin groups were less than model group,the differ-ences were statistically significant （P 〈 0 .0 5 ） . Conclusions Gallic tannic can significantly reduce the liver pathology injury induced by Schistosoma japonicum cercaria,and relieve the degree of liver fibrosis.

关 键 词：没食子鞣酸 日本血吸虫 BABL/C小鼠 肝脏 组织病理学 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]