4种孕妇禁忌中成药对斑马鱼胚胎发育毒性研究  

Study on Developmental Toxicity of Four Chinese Patent Medicines to Zebrafish Embryos

作　　者：陈洁烽 刘彤[1] 黄丹萍[2] 何启迪[1] 陈缵光[1] 姚美村[1] 

机构地区：[1]中山大学药学院,广东广州510006 [2]中山大学附属第三医院,广东广州510000

出　　处：《中国药物警戒》2017年第4期201-204,208,共5页Chinese Journal of Pharmacovigilance

基　　金：广州市科技计划项目(2014J4100171):基于微流控芯片技术和斑马鱼模型的孕妇禁忌中药胚胎毒性快速评价研究

摘　　要：目的研究4种孕妇禁忌中成药妇乐颗粒、妇炎净胶囊、参茸白凤丸、乳癖散结胶囊对斑马鱼胚胎发育的毒性。方法将中成药粉碎成细粉,然后用培养液配制成系列溶液,对斑马鱼胚胎进行培养,分别在24、48、72 h进行显微观察并记录形态变化、死亡胚胎数目和畸形胚胎数目。结果 4种对斑马鱼胚胎的发育均具有明显的毒性,其中妇乐颗粒和妇炎净胶囊则显示出潜在致畸性,可导致斑马鱼胚胎的心脏或尾部水肿,LC50分别为0.743 mg·m L^(-1)和5.23 mg·m L^(-1),EC_(50)分别为0.484 mg·m L^(-1)和3.77 mg·m L^(-1)。结论 4种中成药对斑马鱼胚胎发育具有一定的毒性,斑马鱼模型可用于中成药对胚胎发育毒性影响的研究。Objective To study the developmental toxicity of four pregnancy-contraindicated Chinese patent medicines （Fule granule, Fuyanjing capsule, Shenrongbaifeng pill and Rupisanjie capsule） to zebrafish embryos. Methods Powders of Chinese patent medicines were dissolved and conducted on serial dilutions with E3 medium, respectively. Zebrafish embryos were exposed to these solutions and scored for lethal or teratogenic effects using microscope at 24, 48 and 72 h post fertilization. Results All of the medicines were found to be developmentally toxic. Especially, cardiac edema or tail edema were observed in zebrafish embryos exposed to Fule granule and Fuyanjing capsule, which were proved to be potentially teratogenic. The LC50 and EC50 values for Fule granule were 0.743 mg. mL-1 and 0.484 mg.mL-1, respectively; the corresponding values for Fuyanjing capsule were 5.23 mg- mL＂1 and 3.77 mg. mL-1. Conclusion Developmental toxicity o f 4 Chinese patent medicines was found. It is practicable to study developmental toxicity of Chinese patent medicines using zebrafish model.

关 键 词：中成药 发育毒性 斑马鱼 

分 类 号：R965.3[医药卫生—药理学]