酶联免疫法检测乙肝PreS1抗原及抗体在乙肝诊断中的应用研究  

Study on the application of ELISA in the diagnosis of hepatitis B virus PreS1 antigen and antibody

作　　者：石贺芹 

机构地区：[1]河南省平煤神马医疗集团总医院,平顶山467000

出　　处：《中国实用医刊》2017年第8期45-46,共2页Chinese Journal of Practical Medicine

摘　　要：目的 研究酶联免疫法检测乙肝PreS1抗原及抗体在乙肝诊断中的应用.方法 选取2013年5月至2015年5月192例乙肝＂两对半＂患者的血清作为样本,采用7种常见的检查模式对乙肝PreS1抗原及抗体进行酶联免疫法检测.结果 大三阳乙肝PreS1抗原阴性与阳性分别占16.7%和83.3%;小三阳PreS1抗原阳性占5.7%,分别在单纯表面抗原阳性、表面抗原及核心抗体阳性样本中占3.1%和12.5%.乙肝病毒PreS1抗原阴性组与阳性组的差异比较显示,与HBeAb和HBsAb阳性与否比较差异未见统计学意义（P〉0.05）,与HBcAb、HBeAg、HBsAg阳性与否比较差异有统计学意义（P〈0.05）.提示HBsAg、HBcAb及HBeAg与PreS1抗原呈正相关性.结论 乙肝PreS1抗原及抗体的检测在乙肝诊断方面发挥着重要作用,可用作对＂两对半＂检查结果的补充.Objective To study the application of PreS1 antigen and antibody in diagnosis of hepatitis B by ELISA.Methods From May 2013 to May 2015, 192 patients with hepatitis B were selected as the sample, using 7 kinds of common examination mode to detect hepatitis B PreS1 antigen and antibody by ELISA.Results Big three positive PreS1 antigen negative and positive respectively accounted for 16.7% and 83.3%;Small three positive PreS1 antigen positive accounted for 5.7%, respectively in the simple surface antigen positive, surface antigen and core antigen positive samples accounted for 3.1% and 12.5%.The difference between PreS1 negative group and positive group was not significant （P〉0.05）, compared with HBsAb, HBsAg, HBeAb, HBeAg and HBcAb, and the difference was significant （P〈0.05）.The positive correlation of HBsAg, HBcAb, HBeAg with PreS1 was suggested.Conclusions Hepatitis B PreS1 antigen and antibody detection plays an important role in the diagnosis of hepatitis B, and can be used to supplement the results of ＂two pairs of half＂.

关 键 词：PreS1抗原及抗体 乙肝 酶联免疫法 

分 类 号：R512.62[医药卫生—内科学]